دورية أكاديمية
أعرض في EDS

Performance of IS6110-LAMP assay for detection of Mycobacterium tuberculosis complex in blood and urine samples from patients with extrapulmonary tuberculosis.

التفاصيل البيبلوغرافية
العنوان: Performance of IS6110-LAMP assay for detection of Mycobacterium tuberculosis complex in blood and urine samples from patients with extrapulmonary tuberculosis.
المؤلفون: Araújo RM; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil. Electronic address: araujo.rmartins@gmail.com., Montenegro RA; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Peixoto ADS; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Silva LLS; Federal University of Pernambuco, Recife, Pernambuco, Brazil., da Costa RMPJ; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Carvalho-Silva WHV; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Mello DC; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Diniz GTN; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Silva MALD; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Melo FL; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Montenegro LML; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Schindler HC; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil.
المصدر: Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2023 Dec; Vol. 143, pp. 102423. Date of Electronic Publication: 2023 Oct 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Edinburgh, New York : Churchill Livingstone, c2001-
مواضيع طبية MeSH: Mycobacterium tuberculosis*/genetics , Tuberculosis, Extrapulmonary*, Humans ; Leukocytes, Mononuclear ; DNA
مستخلص: Background: The diagnosis of extrapulmonary tuberculosis (EPTB) shows numerous difficulties because of non-specific symptomatology and low sensitivity of conventional methods. Loop-mediated isothermal amplification (LAMP) is a fast and low-cost technique, which can amplify under isothermal conditions an amount of target DNA copies into approximately a billion copies.
Objective: The present study aimed to evaluate a IS6110-LAMP system for Mycobacterium tuberculosis detection in blood and urine samples from patients with EPTB.
Methods: The collected samples (n = 122) were stratified in two groups: Group EPTB - patient samples with confirmed EPTB (n = 61); Group non-TB - patient samples without TB (n = 61). The urine samples underwent decontamination, and the components of blood samples were separated (plasma and PBMC). DNA extractions were performed in all biological samples followed by IS6110-LAMP assay technique. The detection limit was evaluated through dilution curves (1:10) using Mtb reference strain (H37Rv) genomic DNA.
Findings: The detection limit of IS6110-LAMP was 10 fg/μL (∼10-20 bacilli/μL). The IS6110-LAMP technique sensitivity and specificity were 95.65 % and 79.25 %, respectively, with a general kappa agreement index of 0.762.
Main Conclusions: Based on these results, IS6110-LAMP test showed considerable diagnostic parameters, being able to aid in the speed and accuracy of the final EPTB diagnosis.
Competing Interests: Conflict of interest The authors declared no conflicts of interest.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: EPTB; Loop-mediated isothermal amplification; Molecular test; Mtb; Tuberculosis diagnosis
المشرفين على المادة: 9007-49-2 (DNA)
SCR Protocol: LAMP assay
تواريخ الأحداث: Date Created: 20240105 Date Completed: 20240108 Latest Revision: 20240328
رمز التحديث: 20240329
DOI: 10.1016/j.tube.2023.102423
PMID: 38180027
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-281X
DOI:10.1016/j.tube.2023.102423