دورية أكاديمية
Performance of IS6110-LAMP assay for detection of Mycobacterium tuberculosis complex in blood and urine samples from patients with extrapulmonary tuberculosis.
العنوان: | Performance of IS6110-LAMP assay for detection of Mycobacterium tuberculosis complex in blood and urine samples from patients with extrapulmonary tuberculosis. |
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المؤلفون: | Araújo RM; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil. Electronic address: araujo.rmartins@gmail.com., Montenegro RA; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Peixoto ADS; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Silva LLS; Federal University of Pernambuco, Recife, Pernambuco, Brazil., da Costa RMPJ; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Carvalho-Silva WHV; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Mello DC; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Diniz GTN; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Silva MALD; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Melo FL; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Montenegro LML; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil., Schindler HC; Aggeu Magalhães Institute - FIOCRUZ/PE, Recife, Pernambuco, Brazil. |
المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2023 Dec; Vol. 143, pp. 102423. Date of Electronic Publication: 2023 Oct 20. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
مواضيع طبية MeSH: | Mycobacterium tuberculosis*/genetics , Tuberculosis, Extrapulmonary*, Humans ; Leukocytes, Mononuclear ; DNA |
مستخلص: | Background: The diagnosis of extrapulmonary tuberculosis (EPTB) shows numerous difficulties because of non-specific symptomatology and low sensitivity of conventional methods. Loop-mediated isothermal amplification (LAMP) is a fast and low-cost technique, which can amplify under isothermal conditions an amount of target DNA copies into approximately a billion copies. Objective: The present study aimed to evaluate a IS6110-LAMP system for Mycobacterium tuberculosis detection in blood and urine samples from patients with EPTB. Methods: The collected samples (n = 122) were stratified in two groups: Group EPTB - patient samples with confirmed EPTB (n = 61); Group non-TB - patient samples without TB (n = 61). The urine samples underwent decontamination, and the components of blood samples were separated (plasma and PBMC). DNA extractions were performed in all biological samples followed by IS6110-LAMP assay technique. The detection limit was evaluated through dilution curves (1:10) using Mtb reference strain (H37Rv) genomic DNA. Findings: The detection limit of IS6110-LAMP was 10 fg/μL (∼10-20 bacilli/μL). The IS6110-LAMP technique sensitivity and specificity were 95.65 % and 79.25 %, respectively, with a general kappa agreement index of 0.762. Main Conclusions: Based on these results, IS6110-LAMP test showed considerable diagnostic parameters, being able to aid in the speed and accuracy of the final EPTB diagnosis. Competing Interests: Conflict of interest The authors declared no conflicts of interest. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
فهرسة مساهمة: | Keywords: EPTB; Loop-mediated isothermal amplification; Molecular test; Mtb; Tuberculosis diagnosis |
المشرفين على المادة: | 9007-49-2 (DNA) |
SCR Protocol: | LAMP assay |
تواريخ الأحداث: | Date Created: 20240105 Date Completed: 20240108 Latest Revision: 20240328 |
رمز التحديث: | 20240329 |
DOI: | 10.1016/j.tube.2023.102423 |
PMID: | 38180027 |
قاعدة البيانات: | MEDLINE |
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