دورية أكاديمية
cAMP regulates the progesterone receptor gene expression through the protein kinase A pathway during decidualization in human immortalized endometrial stromal cells.
| العنوان: | cAMP regulates the progesterone receptor gene expression through the protein kinase A pathway during decidualization in human immortalized endometrial stromal cells. |
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| المؤلفون: | Retis-Resendiz AM; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., Cid-Cruz Y; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., Velázquez-Hernández DM; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., Romero-Reyes J; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., León-Juárez M; Departamento de Inmunobioquímica, Instituto Nacional de Perinatología (INPer), Mexico City 11000, Mexico., García-Gómez E; Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT)-Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., Camacho-Arroyo I; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico., Vázquez-Martínez ER; Unidad de Investigación en Reproducción Humana, Instituto Nacional de Perinatología (INPer)-Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Mexico City 11000, Mexico. Electronic address: vamer@comunidad.unam.mx. |
| المصدر: | Steroids [Steroids] 2024 Mar; Vol. 203, pp. 109363. Date of Electronic Publication: 2024 Jan 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0404536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5867 (Electronic) Linking ISSN: 0039128X NLM ISO Abbreviation: Steroids Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Elsevier Original Publication: San Francisco. |
| مواضيع طبية MeSH: | Decidua*/metabolism , Receptors, Progesterone*/genetics , Receptors, Progesterone*/metabolism, Pregnancy ; Female ; Humans ; Cyclic AMP-Dependent Protein Kinases/genetics ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Cyclic AMP-Dependent Protein Kinases/pharmacology ; Endometrium/metabolism ; Progesterone/pharmacology ; Progesterone/metabolism ; Cyclic AMP/metabolism ; Stromal Cells/metabolism ; Gene Expression ; Cells, Cultured |
| مستخلص: | Decidualization, a crucial process for successful pregnancy establishment and maintenance, involves endometrial stromal cell differentiation. This process is orchestrated by estradiol (E2), progesterone, and other stimuli that increase intracellular cyclic adenosine monophosphate (cAMP) levels. The intracellular progesterone receptor (PR), encoded by the PGR gene, has a key role in decidualization. This study aimed to understand the role of sex steroids and cAMP in regulating PGR expression during the in vitro decidualization of the human immortalized endometrial stromal cell line, T-HESC. We subjected the cells to individual and combined treatments of E2, medroxyprogesterone (MPA), and cAMP. Additionally, we treated cells with PR and estrogen receptor antagonists and a protein kinase A (PKA) inhibitor. We evaluated the expression of PGR isoforms and decidualization-associated genes by RT-qPCR. Our findings revealed that cAMP induced PGR-B and PGR-AB expression by activating the PKA signaling pathway, while MPA downregulated their expression through the PR. Furthermore, downstream genes involved in decidualization, such as those coding for prolactin (PRL), insulin-like growth factor-binding protein-1 (IGFBP1), and Dickkopf-1 (DKK1), exhibited positive regulation via the cAMP-PKA pathway. Remarkably, MPA-activated PR signaling induced the expression of IGFBP1 and DKK1 but inhibited that of PRL. In conclusion, we have demonstrated that the PKA signaling pathway induces PGR gene expression during in vitro decidualization of the T-HESC human endometrial stromal cell line. This study has unraveled some of the intricate regulatory mechanisms governing PGR expression during this fundamental process for implantation and pregnancy maintenance. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cyclic adenosine monophosphate; Decidualization; Endometrial stromal cells; Endometrium; Progesterone receptor gene |
| المشرفين على المادة: | 0 (Receptors, Progesterone) EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) 4G7DS2Q64Y (Progesterone) E0399OZS9N (Cyclic AMP) |
| تواريخ الأحداث: | Date Created: 20240105 Date Completed: 20240214 Latest Revision: 20240214 |
| رمز التحديث: | 20240214 |
| DOI: | 10.1016/j.steroids.2024.109363 |
| PMID: | 38182066 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-5867 |
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| DOI: | 10.1016/j.steroids.2024.109363 |