دورية أكاديمية
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Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants.

التفاصيل البيبلوغرافية
العنوان: Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants.
المؤلفون: Stoneman MR; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA., McCoy VE; Department of Geosciences, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA. mccoyv@uwm.edu.; School of Geography, Geology, and the Environment, University of Leicester, Leicester, LE1 7RH, UK. mccoyv@uwm.edu., Gee CT; Institute of Geosciences, Division of Paleontology, University of Bonn, Nussallee 8, 53115, Bonn, Germany., Bober KMM; Department of Geosciences, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA., Raicu V; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI, 53211, USA. vraicu@uwm.edu.
المصدر: Communications biology [Commun Biol] 2024 Jan 06; Vol. 7 (1), pp. 53. Date of Electronic Publication: 2024 Jan 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-
مواضيع طبية MeSH: Ecosystem* , Fossils*, Microscopy, Fluorescence ; Amber ; Cellulose ; Fluorescent Dyes ; Ionophores
مستخلص: Fluorescence emission is common in plants. While fluorescence microscopy has been widely used to study living plants, its application in quantifying the fluorescence of fossil plants has been limited. Fossil plant fluorescence, from original fluorophores or formed during fossilization, can offer valuable insights into fluorescence in ancient plants and fossilization processes. In this work, we utilize two-photon fluorescence microspectroscopy to spatially and spectrally resolve the fluorescence emitted by amber-embedded plants, leaf compressions, and silicified wood. The advanced micro-spectroscope utilized, with its pixel-level spectral resolution and line-scan excitation capabilities, allows us to collect comprehensive excitation and emission spectra with high sensitivity and minimal laser damage to the specimens. By applying linear spectral unmixing to the spectrally resolved fluorescence images, we can differentiate between (a) the matrix and (b) the materials that comprise the fossil. Our analysis suggests that the latter correspond to durable tissues such as lignin and cellulose. Additionally, we observe potential signals from chlorophyll derivatives/tannins, although minerals may have contributed to this. This research opens doors to exploring ancient ecosystems and understanding the ecological roles of fluorescence in plants throughout time. Furthermore, the protocols developed herein can also be applied to analyze non-plant fossils and biological specimens.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 1919670 National Science Foundation (NSF); 348043586 Deutsche Forschungsgemeinschaft (German Research Foundation)
المشرفين على المادة: 0 (Amber)
9004-34-6 (Cellulose)
0 (Fluorescent Dyes)
0 (Ionophores)
تواريخ الأحداث: Date Created: 20240106 Date Completed: 20240108 Latest Revision: 20240202
رمز التحديث: 20240202
مُعرف محوري في PubMed: PMC10771488
DOI: 10.1038/s42003-024-05763-z
PMID: 38184735
قاعدة البيانات: MEDLINE
الوصف
تدمد:2399-3642
DOI:10.1038/s42003-024-05763-z