دورية أكاديمية
أعرض في EDS

The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2.

التفاصيل البيبلوغرافية
العنوان: The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2.
المؤلفون: Al-Qaoud KM; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Obeidat YM; Department of Electronics Engineering, Hijjawi Faculty for Engineering Technology, Yarmouk University, Irbid, Jordan. yusra.obeidat@yu.edu.jo., Al-Omari T; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Okour M; Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan., Al-Omari MM; Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan., Ahmad MI; Rawgene Biotech, Umm Khelad St. 33, Amman, Jordan.; Atlas Medical, Sahab Industrial Area, Amman, Jordan.; Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal., Alshadfan R; Rawgene Biotech, Umm Khelad St. 33, Amman, Jordan.; Atlas Medical, Sahab Industrial Area, Amman, Jordan., Rawashdeh AM; Department of Chemistry, Faculty of Science, Yarmouk University, Irbid, Jordan.
المصدر: Scientific reports [Sci Rep] 2024 Jan 07; Vol. 14 (1), pp. 748. Date of Electronic Publication: 2024 Jan 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Biosensing Techniques* , COVID-19*/diagnosis , Blood Group Antigens*, Animals ; Chickens ; Immunoassay ; RNA, Viral ; SARS-CoV-2 ; Antibodies
مستخلص: This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
(© 2024. The Author(s).)
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معلومات مُعتمدة: Project No. MPH/2/14/2020 the Scientific Research and Innovation Fund (SRIF)
المشرفين على المادة: 0 (IgY)
0 (RNA, Viral)
0 (Antibodies)
0 (Blood Group Antigens)
تواريخ الأحداث: Date Created: 20240107 Date Completed: 20240109 Latest Revision: 20240110
رمز التحديث: 20240111
مُعرف محوري في PubMed: PMC10772103
DOI: 10.1038/s41598-023-50501-w
PMID: 38185704
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-50501-w