دورية أكاديمية
أعرض في EDS

Proteome profile of the cerebellum from α7 nicotinic acetylcholine receptor deficient mice.

التفاصيل البيبلوغرافية
العنوان: Proteome profile of the cerebellum from α7 nicotinic acetylcholine receptor deficient mice.
المؤلفون: Caban KM; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, München, Germany., Seßenhausen P; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig Maximilian University of Munich, Planegg-Martinsried, Germany., Stöckl JB; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, München, Germany., Popper B; Biomedical Center (BMC), Core Facility Animal Models, Faculty of Medicine, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany., Mayerhofer A; Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, Ludwig Maximilian University of Munich, Planegg-Martinsried, Germany., Fröhlich T; Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, München, Germany.
المصدر: Proteomics [Proteomics] 2024 May; Vol. 24 (10), pp. e2300384. Date of Electronic Publication: 2024 Jan 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101092707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-9861 (Electronic) Linking ISSN: 16159853 NLM ISO Abbreviation: Proteomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim, Germany : Wiley-VCH,
مواضيع طبية MeSH: alpha7 Nicotinic Acetylcholine Receptor*/metabolism , alpha7 Nicotinic Acetylcholine Receptor*/genetics , Cerebellum*/metabolism , Proteome*/metabolism , Proteome*/analysis, Animals ; Mice ; Chromatography, Liquid/methods ; Liver/metabolism ; Mice, Knockout ; Proteomics/methods ; Tandem Mass Spectrometry
مستخلص: The alpha7 nicotinic acetylcholine receptor (α7 nAChR; CHRNA7) is expressed in the nervous system and in non-neuronal tissues. Within the central nervous system, it is involved in various cognitive and sensory processes such as learning, attention, and memory. It is also expressed in the cerebellum, where its roles are; however, not as well understood as in the other brain regions. To investigate the consequences of absence of CHRNA7 on the cerebellum proteome, we performed a quantitative nano-LC-MS/MS analysis of samples from CHRNA7 knockout (KO) mice and corresponding wild type (WT) controls. Liver, an organ which does not express this receptor, was analyzed, in comparison. While the liver proteome remained relatively unaltered (three proteins more abundant in KOs), 90 more and 20 less abundant proteins were detected in the cerebellum proteome of the KO mice. The gene ontology analysis of the differentially abundant proteins indicates that the absence of CHRNA7 leads to alterations in the glutamatergic system and myelin sheath in the cerebellum. In conclusion, our dataset provides new insights in the role of CHRNA7 in the cerebellum, which may serve as a basis for future in depth-investigations.
(© 2024 The Authors. PROTEOMICS published by Wiley‐VCH GmbH.)
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معلومات مُعتمدة: 432434245 Deutsche Forschungsgemeinschaft
فهرسة مساهمة: Keywords: acetylcholine; cerebellum; mass spectrometry; nicotinic receptor
المشرفين على المادة: 0 (alpha7 Nicotinic Acetylcholine Receptor)
0 (Chrna7 protein, mouse)
0 (Proteome)
تواريخ الأحداث: Date Created: 20240107 Date Completed: 20240514 Latest Revision: 20240712
رمز التحديث: 20240712
DOI: 10.1002/pmic.202300384
PMID: 38185761
قاعدة البيانات: MEDLINE
الوصف
تدمد:1615-9861
DOI:10.1002/pmic.202300384