Distinct cellular expression and subcellular localization of Kv2 voltage-gated K + channel subtypes in dorsal root ganglion neurons conserved between mice and humans.
| العنوان: | Distinct cellular expression and subcellular localization of Kv2 voltage-gated K + channel subtypes in dorsal root ganglion neurons conserved between mice and humans. |
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| المؤلفون: | Stewart RG; Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616, USA., Camacena M; Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616, USA., Copits BA; Washington University Pain Center, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA., Sack JT; Department of Physiology and Membrane Biology, University of California Davis, Davis, CA 95616, USA.; Department of Anesthesiology and Pain Medicine, University of California Davis, Davis, CA 95616, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Dec 24. Date of Electronic Publication: 2023 Dec 24. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | The distinct organization of Kv2 voltage-gated potassium channels on and near the cell body of brain neurons enables their regulation of action potentials and specialized membrane contact sites. Somatosensory neurons have a pseudounipolar morphology and transmit action potentials from peripheral nerve endings through axons that bifurcate to the spinal cord and the cell body within ganglia including the dorsal root ganglia (DRG). Kv2 channels regulate action potentials in somatosensory neurons, yet little is known about where Kv2 channels are located. Here we define the cellular and subcellular localization of the Kv2 paralogs, Kv2.1 and Kv2.2, in DRG somatosensory neurons with a panel of antibodies, cell markers, and genetically modified mice. We find that relative to spinal cord neurons, DRG neurons have similar levels of detectable Kv2.1, and higher levels of Kv2.2. In older mice, detectable Kv2.2 remains similar while detectable Kv2.1 decreases. Both Kv2 subtypes adopt clustered subcellular patterns that are distinct from central neurons. Most DRG neurons co-express Kv2.1 and Kv2.2, although neuron subpopulations show preferential expression of Kv2.1 or Kv2.2. We find that Kv2 protein expression and subcellular localization is similar between mouse and human DRG neurons. We conclude that the organization of both Kv2 channels is consistent with physiological roles in the somata and stem axons of DRG neurons. The general prevalence of Kv2.2 in DRG as compared to central neurons and the enrichment of Kv2.2 relative to detectable Kv2.1, in older mice, proprioceptors, and axons suggest more widespread roles for Kv2.2 in DRG neurons. Competing Interests: Conflict of interest statement: The authors declare no competing conflicts of interest. |
| التعليقات: | Update in: J Comp Neurol. 2024 Feb;532(2):e25575. (PMID: 38335058) |
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| معلومات مُعتمدة: | R01 NS096317 United States NS NINDS NIH HHS |
| فهرسة مساهمة: | Keywords: Dorsal root ganglion; Kv2.1; Kv2.2; Somatosensory neurons; Voltage gated ion channels |
| تواريخ الأحداث: | Date Created: 20240108 Latest Revision: 20240426 |
| رمز التحديث: | 20240426 |
| مُعرف محوري في PubMed: | PMC10769185 |
| DOI: | 10.1101/2023.03.01.530679 |
| PMID: | 38187582 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2023.03.01.530679 |
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