دورية أكاديمية
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Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization of Bacillus subtilis Cells and Spores.

التفاصيل البيبلوغرافية
العنوان: Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization of Bacillus subtilis Cells and Spores.
المؤلفون: Huang Y; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.; Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Swarge BN; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.; Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Roseboom W; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Bleeker JD; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Brul S; Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands., Setlow P; Department of Molecular Biology and Biophysics, UConn Health, Farmington, Connecticut 06030-3305, United States., Kramer G; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.
المصدر: Journal of proteome research [J Proteome Res] 2024 Feb 02; Vol. 23 (2), pp. 596-608. Date of Electronic Publication: 2024 Jan 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, c2002-
مواضيع طبية MeSH: Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Proteomics*, Spores, Bacterial/genetics ; Spores, Bacterial/metabolism ; Methanol ; Water/metabolism ; Ethanol/metabolism
مستخلص: Reliable and comprehensive multi-omics analysis is essential for researchers to understand and explore complex biological systems more completely. Bacillus subtilis (B. subtilis) is a model organism for Gram-positive spore-forming bacteria, and in-depth insight into the physiology and molecular basis of spore formation and germination in this organism requires advanced multilayer molecular data sets generated from the same sample. In this study, we evaluated two monophasic methods for polar and nonpolar compound extraction (acetonitrile/methanol/water; isopropanol/water, and 60% ethanol) and two biphasic methods (chloroform/methanol/water, and methyl tert-butyl ether/methanol/water) on coefficients of variation of analytes, identified metabolite composition, and the quality of proteomics profiles. The 60% EtOH protocol proved to be the easiest in sample processing and was more amenable to automation. Collectively, we annotated 505 and 484 metabolites and identified 1665 and 1562 proteins in B. subtilis vegetative cells and spores, respectively. We also show differences between vegetative cells and spores from a multi-omics perspective and demonstrate that an integrative multi-omics analysis can be implemented from one sample using the 60% EtOH protocol. The results obtained by the 60% EtOH protocol provide comprehensive insight into differences in the metabolic and protein makeup of B. subtilis vegetative cells and spores.
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فهرسة مساهمة: Keywords: Bacillus subtilis; metabolomics; multi-omics; proteomics; sample extraction; spores
المشرفين على المادة: Y4S76JWI15 (Methanol)
059QF0KO0R (Water)
3K9958V90M (Ethanol)
تواريخ الأحداث: Date Created: 20240108 Date Completed: 20240205 Latest Revision: 20240313
رمز التحديث: 20240314
مُعرف محوري في PubMed: PMC10845140
DOI: 10.1021/acs.jproteome.3c00386
PMID: 38190553
قاعدة البيانات: MEDLINE
الوصف
تدمد:1535-3907
DOI:10.1021/acs.jproteome.3c00386