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Analysis of the Morphology of Monocytes and Lymphocytes from COVID-19 Patients Using Low-Voltage Scanning Electronic Microscopy.

التفاصيل البيبلوغرافية
العنوان: Analysis of the Morphology of Monocytes and Lymphocytes from COVID-19 Patients Using Low-Voltage Scanning Electronic Microscopy.
المؤلفون: Velmiskina AA; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia.; St. Petersburg State University, St. Petersburg, Russia., Nikitin YV; S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia., Mikhailovskii VY; St. Petersburg State University, St. Petersburg, Russia., Mosenko SV; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia.; St. Petersburg State University, St. Petersburg, Russia., Anisenkova AY; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia.; St. Petersburg State University, St. Petersburg, Russia., Apalko SV; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia., Sushentseva NN; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia., Scherbak SG; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia.; St. Petersburg State University, St. Petersburg, Russia., Ivanov AM; S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia., Galaktionov NK; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia., Shneider OV; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia., Kondratov KA; St. Petersburg City Hospital No. 40, Sestroretsk, St. Petersburg, Russia. kondratovk.kirill@yandex.ru.; St. Petersburg State University, St. Petersburg, Russia. kondratovk.kirill@yandex.ru.; S. M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, St. Petersburg, Russia. kondratovk.kirill@yandex.ru.
المصدر: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Dec; Vol. 176 (2), pp. 297-302. Date of Electronic Publication: 2024 Jan 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 0372557 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-8221 (Electronic) Linking ISSN: 00074888 NLM ISO Abbreviation: Bull Exp Biol Med Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Springer
Original Publication: New York, Consultants Bureau.
مواضيع طبية MeSH: COVID-19*, Humans ; Monocytes ; SARS-CoV-2 ; Cytokine Release Syndrome ; Microscopy, Electron, Scanning ; Cytokines ; Lymphocytes ; Electronics
مستخلص: Severe course of COVID-19 is largely determined by hyperactivation of the immune system, or cytokine storm, in which immune cells (lymphocytes, monocytes, etc.) play a major role. Using low-voltage scanning electron microscopy, we studied the morphology of lymphocytes and monocytes during cytokine storm. Monocytes and lymphocytes were isolated by fluorescence sorting from the blood of healthy volunteers (n=6) and patients with COVID-19 (n=5) during cytokine storm (IL-6>23 ng/ml, smear positive for SARS-CoV-2). For each patient, 11-32 individual cells were analyzed at magnification of 18-32,000 times. Measurements showed that monocyte size was increased during cytokine storm (p=0.0001).
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
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فهرسة مساهمة: Keywords: COVID-19; cytokine storm; low-voltage scanning electron microscopy; lymphocytes; monocytes
المشرفين على المادة: 0 (Cytokines)
تواريخ الأحداث: Date Created: 20240109 Date Completed: 20240119 Latest Revision: 20240213
رمز التحديث: 20240213
DOI: 10.1007/s10517-024-06011-4
PMID: 38194071
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-8221
DOI:10.1007/s10517-024-06011-4