دورية أكاديمية
Genetic diversity of Toxoplasma gondii in goats and sheep from the Northeast Region of Brazil destined for human consumption.
| العنوان: | Genetic diversity of Toxoplasma gondii in goats and sheep from the Northeast Region of Brazil destined for human consumption. |
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| المؤلفون: | Feitosa TF; Department of Veterinary Medicine, Federal Institute of Paraíba - IFPB, Sousa, Paraíba, ZC 58800-970, Brazil., Vilela VLR; Department of Veterinary Medicine, Federal Institute of Paraíba - IFPB, Sousa, Paraíba, ZC 58800-970, Brazil.; Postgraduate Program in Science and Animal Health, Federal University of Campina Grande - UFCG, Patos, Paraíba, ZC 58708-110, Brazil., Batista SP; Postgraduate Program in Science and Animal Health, Federal University of Campina Grande - UFCG, Patos, Paraíba, ZC 58708-110, Brazil., Silva SS; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, Brazil., Mota RA; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife, Pernambuco, ZC 52171-900, Brazil., Katzer F; Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, Scotland, United Kingdom., Bartley PM; Moredun Research Institute, Pentlands Science Park, Penicuik, EH26 0PZ, Scotland, United Kingdom. |
| المصدر: | Current research in parasitology & vector-borne diseases [Curr Res Parasitol Vector Borne Dis] 2023 Dec 09; Vol. 5, pp. 100163. Date of Electronic Publication: 2023 Dec 09 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 9918226380706676 Publication Model: eCollection Cited Medium: Internet ISSN: 2667-114X (Electronic) Linking ISSN: 2667114X NLM ISO Abbreviation: Curr Res Parasitol Vector Borne Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier B.V., [2021]- |
| مستخلص: | This study aimed to genotype isolates of Toxoplasma gondii obtained from samples of brain, diaphragm and heart of goats and sheep intended for human consumption in the State of Paraíba, Brazil. Tissue samples from 14 animals, goats ( n = 5) and lambs ( n = 9), were sourced from public slaughterhouses in seven cities and bio-assayed in mice. The brains of the mice were utilized for DNA extraction. Genotyping was carried out by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using 10 markers (SAG1, SAG2, SAG3, BTUB, c22-8, PK1, GRA6, L358, c-29-2 and Apico). A total of 10 isolates were fully genotyped (i.e. at all loci), three from goats and seven from sheep, revealing five distinct genotypes: #13 ( n = 4); #48 ( n = 3); #57 ( n = 1); #273 ( n = 1); and one new genotype that had not been previously described. Genotype #13 is frequently found in the Northeast of Brazil and represents a clonal lineage circulating in this region and was the most prevalent genotype identified ( n = 4). Moreover, in the present study genotypes #13, #48, #57, and #273 were documented for the first time in sheep from Brazil, and the novel genotype was isolated from a goat. Our findings align with previous studies on T. gondii from Brazil, where new genotypes are continuously being identified, highlighting a high level of genetic diversity of T. gondii isolates in the country. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Given their role as Co-Editor, Frank Katzer had no involvement in the peer review of this article and has no access to information regarding its peer review. Full responsibility for the editorial process for this article was delegated to Professor Aneta Kostadinova (Editor-in-Chief). (© 2023 The Authors.) |
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| فهرسة مساهمة: | Keywords: Food-borne pathogens; Genotyping; Goats; PCR-RFLP; Sheep; Toxoplasma gondii |
| تواريخ الأحداث: | Date Created: 20240110 Latest Revision: 20240111 |
| رمز التحديث: | 20240111 |
| مُعرف محوري في PubMed: | PMC10775902 |
| DOI: | 10.1016/j.crpvbd.2023.100163 |
| PMID: | 38196495 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2667-114X |
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| DOI: | 10.1016/j.crpvbd.2023.100163 |