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Overexpression of PvWAK3 from seashore paspalum increases salt tolerance in transgenic Arabidopsis via maintenance of ion and ROS homeostasis.

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العنوان: Overexpression of PvWAK3 from seashore paspalum increases salt tolerance in transgenic Arabidopsis via maintenance of ion and ROS homeostasis.
المؤلفون: Li Y; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 22719110@njau.edu.cn., Yang Q; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 2022220001@stu.njau.edu.cn., Huang H; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 9201410129@stu.njau.edu.cn., Guo Y; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 9201410924@stu.njau.edu.cn., Sun Q; Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, China. Electronic address: sunqg@jsafc.edu.cn., Guo Z; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: zfguo@njau.edu.cn., Shi H; College of Grassland Science, Key Laboratory of State Forestry and Grassland Administration on Grass Germplasm Resources Innovation and Utilization in the Middle and Lower Reaches of the Yangtze River, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: shihaifan@njau.edu.cn.
المصدر: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Feb; Vol. 207, pp. 108337. Date of Electronic Publication: 2024 Jan 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier Science
Original Publication: Paris : Gauthier-Villars, c1987-
مواضيع طبية MeSH: Paspalum* , Arabidopsis*/genetics , Arabidopsis Proteins*, Salt Tolerance/genetics ; Reactive Oxygen Species ; Hydrogen Peroxide ; Plants, Genetically Modified/physiology ; Membrane Transport Proteins ; Homeostasis ; Proline ; Gene Expression Regulation, Plant ; Potassium Channels
مستخلص: Seashore paspalum (Paspalum vaginatum O. Swartz) is an important warm-season turfgrass species with extreme salt tolerance, but investigations on its salt tolerance mechanism are limited. A salt induced PvWAK3 from halophyte seashore paspalum was identified in this study. Overexpression of PvWAK3 in Arabidopsis led to increased salt tolerance. Transgenic plants had higher levels of seed germination rate, root length, number of lateral roots, shoot weight, survival rate, F v /F m , ETR, and NPQ compared with the wild type (WT) under salt stress. Na + content was increased and K + content was decreased after salinity treatment, with lower levels of Na + and Na + /K + ratio but higher level of K + in transgenic plants than in WT under salt stress. The improved maintenance of Na + and K + homeostasis was associated with the higher transcript levels of K + -Uptake Permease 4 (KUP4), Potassium Transport 2/3 (AKT2), Salt Overly Sensitive 1 (SOS1) and High-Affinity K + Transporter 5 (HAK5) in transgenic plants compared with WT. Superoxide dismutase (SOD), catalase (CAT) and ascorbate-peroxidase (APX) activities, proline concentration, and P5CS1 transcript were increased after salinity treatment, with higher levels in transgenic lines compared with WT, which led to reduced accumulation of O 2 · - and H 2 O 2 under salt stress. It is suggested that PvWAK3 regulates salt tolerance positively, which is associated with promoted Na + and K + homeostasis, activated antioxidant enzymes, and proline biosynthesis under salt stress.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Haifan Shi reports financial support was provided by National Natural Science Foundation of China and Fundamental Research Funds for the Central Universities. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
فهرسة مساهمة: Keywords: Antioxidant defense system; Ion homeostasis; Salt tolerance; Seashore paspalum; Wall-associated kinase
المشرفين على المادة: 0 (Reactive Oxygen Species)
BBX060AN9V (Hydrogen Peroxide)
0 (Membrane Transport Proteins)
9DLQ4CIU6V (Proline)
0 (AKT2 protein, Arabidopsis)
0 (Potassium Channels)
0 (Arabidopsis Proteins)
تواريخ الأحداث: Date Created: 20240110 Date Completed: 20240318 Latest Revision: 20240318
رمز التحديث: 20240318
DOI: 10.1016/j.plaphy.2024.108337
PMID: 38199027
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2690
DOI:10.1016/j.plaphy.2024.108337