دورية أكاديمية
أعرض في EDS

Serpin-4 Negatively Regulates Prophenoloxidase Activation and Antimicrobial Peptide Synthesis in the Silkworm, Bombyx mori .

التفاصيل البيبلوغرافية
العنوان: Serpin-4 Negatively Regulates Prophenoloxidase Activation and Antimicrobial Peptide Synthesis in the Silkworm, Bombyx mori .
المؤلفون: Qie X; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Yan X; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Wang W; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Liu Y; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Zhang L; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Hao C; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China., Lu Z; Department of Entomology, College of Plant Protection, Northwest A&F University, Xianyang 712100, China., Ma L; Department of Plant Protection, College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Dec 25; Vol. 25 (1). Date of Electronic Publication: 2023 Dec 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Serpins*/genetics , Bombyx* , Cecropins* , Catechol Oxidase* , Enzyme Precursors*, Animals ; Serine Endopeptidases ; Serine Proteases/genetics ; Chromosomal Proteins, Non-Histone
مستخلص: The prophenoloxidase (PPO) activation and Toll antimicrobial peptide synthesis pathways are two critical immune responses in the insect immune system. The activation of these pathways is mediated by the cascade of serine proteases, which is negatively regulated by serpins. In this study, we identified a typical serpin, BmSerpin-4, in silkworms, whose expression was dramatically up-regulated in the fat body and hemocytes after bacterial infections. The pre-injection of recombinant BmSerpin-4 remarkably decreased the antibacterial activity of the hemolymph and the expression of the antimicrobial peptides (AMPs) gloverin-3 , cecropin-D , cecropin-E , and moricin in the fat body under Micrococcus luteus and Yersinia pseudotuberculosis serotype O: 3 ( YP III ) infection. Meanwhile, the inhibition of systemic melanization, PO activity, and PPO activation by BmSerpin-4 was also observed. Hemolymph proteinase 1 (HP1), serine protease 2 (SP2), HP6, and SP21 were predicted as the candidate target serine proteases for BmSerpin-4 through the analysis of residues adjacent to the scissile bond and comparisons of orthologous genes in Manduca sexta . This suggests that HP1, SP2, HP6, and SP21 might be essential in the activation of the serine protease cascade in both the Toll and PPO pathways in silkworms. Our study provided a comprehensive characterization of BmSerpin-4 and clues for the further dissection of silkworm PPO and Toll activation signaling.
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معلومات مُعتمدة: 32200391, 32300403 National Natural Science Foundation of China; 202103021223125, 202203021212469 Natural Science Foundation of Shanxi Province; 2023YQPYGC05 the Distinguished and Excellent Young Scholar Cultivation Project of Shanxi Agricultural University; 2020BQ71, 2022BQ25 Science and Technology Innovation Foundation of Shanxi Agricultural University
فهرسة مساهمة: Keywords: Bombyx mori; antimicrobial peptide; immune system; prophenoloxidase; serpin
المشرفين على المادة: EC 1.10.3.- (pro-phenoloxidase)
0 (Serpins)
0 (Cecropins)
EC 3.4.21.- (Serine Endopeptidases)
EC 3.4.- (Serine Proteases)
0 (Chromosomal Proteins, Non-Histone)
EC 1.10.3.1 (Catechol Oxidase)
0 (Enzyme Precursors)
تواريخ الأحداث: Date Created: 20240111 Date Completed: 20240112 Latest Revision: 20240113
رمز التحديث: 20240113
مُعرف محوري في PubMed: PMC10778760
DOI: 10.3390/ijms25010313
PMID: 38203484
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25010313