دورية أكاديمية
أعرض في EDS

Metabolomics Integration in Assisted Reproductive Technologies for Enhanced Embryo Selection beyond Morphokinetic Analysis.

التفاصيل البيبلوغرافية
العنوان: Metabolomics Integration in Assisted Reproductive Technologies for Enhanced Embryo Selection beyond Morphokinetic Analysis.
المؤلفون: Pinto S; Centre for Reproductive Genetics Alberto Barros, 4100-012 Porto, Portugal., Guerra-Carvalho B; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal., Crisóstomo L; Institute of Biomedicine, University of Turku, 20014 Turku, Finland., Rocha A; CECA/ICETA-Centro de Estudos de Ciência Animal, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4200-135 Porto, Portugal., Barros A; Centre for Reproductive Genetics Alberto Barros, 4100-012 Porto, Portugal.; i3S-Instituto de Investigação e Inovação em Saúde, University of Porto, 4200-135 Porto, Portugal.; Department of Pathology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal., Alves MG; Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal., Oliveira PF; LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Dec 29; Vol. 25 (1). Date of Electronic Publication: 2023 Dec 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Glutamine* , Reproductive Techniques, Assisted*, Female ; Pregnancy ; Humans ; Pyruvic Acid ; Alanine ; Lactic Acid ; Acetates
مستخلص: Embryo quality evaluation during in vitro development is a crucial factor for the success of assisted reproductive technologies (ARTs). However, the subjectivity inherent in the morphological evaluation by embryologists can introduce inconsistencies that impact the optimal embryo choice for transfer. To provide a more comprehensive evaluation of embryo quality, we undertook the integration of embryo metabolomics alongside standardized morphokinetic classification. The culture medium of 55 embryos (derived from 21 couples undergoing ICSI) was collected at two timepoints (days 3 and 5). Samples were split into Good (n = 29), Lagging (n = 19), and Bad (n = 10) according to embryo morphokinetic evaluation. Embryo metabolic performance was assessed by monitoring the variation in specific metabolites (pyruvate, lactate, alanine, glutamine, acetate, formate) using 1 H-NMR. Adjusted metabolite differentials were observed during the first 3 days of culture and found to be discriminative of embryo quality at the end of day 5. Pyruvate, alanine, glutamine, and acetate were major contributors to this discrimination. Good and Lagging embryos were found to export and accumulate pyruvate and glutamine in the first 3 days of culture, while Bad embryos consumed them. This suggests that Bad embryos have less active metabolic activity than Good and Lagging embryos, and these two metabolites are putative biomarkers for embryo quality. This study provides a more comprehensive evaluation of embryo quality and can lead to improvements in ARTs by enabling the selection of the best embryos. By combining morphological assessment and metabolomics, the selection of high-quality embryos with the potential to result in successful pregnancies may become more accurate and consistent.
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معلومات مُعتمدة: Pedro F. Oliveira (CEECINST/00026/2018), Marco G. Alves (2021.03439.CEECIND), LAQV-REQUIMTE (UIDB/50006/2020); and co-funded by the European Union Regional Development Funds (FEDER) through the COMPETE/QREN, FSE/POPH, and POCI-COMPETE 2020 (POCI-01-0145-F Fundação para a Ciência e Tecnologia
فهرسة مساهمة: Keywords: embryo metabolomics; embryo quality evaluation; glutamine; morphokinetic assessment; pyruvate
المشرفين على المادة: 0RH81L854J (Glutamine)
8558G7RUTR (Pyruvic Acid)
OF5P57N2ZX (Alanine)
33X04XA5AT (Lactic Acid)
0 (Acetates)
تواريخ الأحداث: Date Created: 20240111 Date Completed: 20240112 Latest Revision: 20240113
رمز التحديث: 20240113
مُعرف محوري في PubMed: PMC10778973
DOI: 10.3390/ijms25010491
PMID: 38203668
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25010491