دورية أكاديمية
Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis.
العنوان: | Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis. |
---|---|
المؤلفون: | Vander Elst N; Laboratory of Gene Technology, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 21, 3001, Heverlee, Belgium. Niels.VanderElst@ugent.be.; Laboratory of Applied Biotechnology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium. Niels.VanderElst@ugent.be.; Laboratory of Biochemistry, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium. Niels.VanderElst@ugent.be., Bellemans J; Laboratory of Applied Biotechnology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium.; Laboratory of Biochemistry, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium., Lavigne R; Laboratory of Gene Technology, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 21, 3001, Heverlee, Belgium., Briers Y; Laboratory of Applied Biotechnology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium., Meyer E; Laboratory of Biochemistry, Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium. Evelyne.Meyer@ugent.be. |
المصدر: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Dec; Vol. 108 (1), pp. 118. Date of Electronic Publication: 2024 Jan 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer International, c1984- |
مواضيع طبية MeSH: | Streptococcal Infections*/drug therapy , Streptococcal Infections*/veterinary , Mastitis, Bovine*/drug therapy , Streptococcus* , Endopeptidases*, Female ; Animals ; Cattle ; Mice ; Cloxacillin/therapeutic use ; Lactation ; Disease Models, Animal |
مستخلص: | Streptococcus uberis frequently causes bovine mastitis, an infectious udder disease with significant economic implications for dairy cows. Conventional antibiotics, such as cloxacillin, sometimes have limited success in eliminating S. uberis as a stand-alone therapy. To address this challenge, the study objective was to investigate the VersaTile engineered endolysin NC5 as a supplemental therapy to cloxacillin in a mouse model of bovine S. uberis mastitis. NC5 was previously selected based on its intracellular killing and biofilm eradicating activity. To deliver preclinical proof-of-concept of this supplemental strategy, lactating mice were intramammarily infected with a bovine S. uberis field isolate and subsequently treated with cloxacillin (30.0 μg) combined with either a low (23.5 μg) or high (235.0 μg) dose of NC5. An antibiotic monotherapy group, as well as placebo treatment, was included as controls. Two types of responders were identified: fast (n = 17), showing response after 4-h treatment, and slow (n = 10), exhibiting no clear response at 4 h post-treatment across all groups. The high-dose combination therapy in comparison with placebo treatment impacted the hallmarks of mastitis in the fast responders by reducing (i) the bacterial load 13,000-fold (4.11 ± 0.78 Δlog (© 2024. The Author(s).) |
References: | Vet Res. 2023 Mar 27;54(1):28. (PMID: 36973819) J Vet Pharmacol Ther. 2019 Nov;42(6):693-706. (PMID: 31553070) Biomol Ther (Seoul). 2023 Mar 1;31(2):141-147. (PMID: 36788654) J Appl Microbiol. 2022 Mar;132(3):1751-1759. (PMID: 34800320) J Pathol Inform. 2012;3:25. (PMID: 22934238) Front Immunol. 2022 Oct 21;13:1031785. (PMID: 36341445) Viruses. 2021 Apr 15;13(4):. (PMID: 33920965) Antibiotics (Basel). 2023 May 12;12(5):. (PMID: 37237804) Front Microbiol. 2020 Jan 23;11:7. (PMID: 32038593) Sci Rep. 2016 Apr 28;6:25063. (PMID: 27121552) Vet Res. 2016 Jan 07;47:13. (PMID: 26738804) Antibiotics (Basel). 2020 Sep 19;9(9):. (PMID: 32961696) J Vet Med. 2014;2014:659801. (PMID: 26464939) Front Immunol. 2017 Jul 24;8:833. (PMID: 28791009) Int Breastfeed J. 2015 Apr 06;10:12. (PMID: 25848399) Appl Microbiol Biotechnol. 2015 Oct;99(20):8475-86. (PMID: 25895090) J Dairy Res. 1993 Feb;60(1):19-29. (PMID: 8436664) Sci Adv. 2020 Jun 03;6(23):eaaz1136. (PMID: 32537492) Microb Biotechnol. 2023 Dec;16(12):2367-2386. (PMID: 37853918) Int J Antimicrob Agents. 2015 Jul;46(1):13-20. (PMID: 26003836) Front Immunol. 2018 Jun 25;9:1487. (PMID: 29988549) J Dairy Sci. 2013;96(11):7082-7087. (PMID: 24054294) Vet Q. 2006 Mar;28(1):2-13. (PMID: 16605156) J Dairy Res. 2017 May;84(2):198-201. (PMID: 28524019) Transbound Emerg Dis. 2018 May;65 Suppl 1:166-185. (PMID: 29083115) Front Immunol. 2018 May 28;9:1143. (PMID: 29892291) Commun Biol. 2020 Sep 17;3(1):512. (PMID: 32943734) Antimicrob Agents Chemother. 2010 Apr;54(4):1603-12. (PMID: 20086153) Oncoimmunology. 2022 Jul 22;11(1):2103277. (PMID: 35898705) Vet Microbiol. 2018 Jan;213:73-81. (PMID: 29292007) Vet Res. 2003 Sep-Oct;34(5):521-64. (PMID: 14556694) Microb Pathog. 2006 Jun;40(6):279-85. (PMID: 16678381) Front Vet Sci. 2020 Nov 09;7:576583. (PMID: 33240956) Semin Immunol. 2021 Apr;54:101516. (PMID: 34728120) Appl Environ Microbiol. 2012 Apr;78(7):2297-305. (PMID: 22286996) PLoS One. 2009;4(3):e4729. (PMID: 19266100) Toxicol Pathol. 2010 Aug;38(5):681-90. (PMID: 20562325) Front Microbiol. 2023 Mar 24;14:1126896. (PMID: 37032878) J Pharm Sci. 2008 Jun;97(6):2041-4. (PMID: 17847072) Res Vet Sci. 2022 Mar;143:41-49. (PMID: 34973538) |
معلومات مُعتمدة: | 1.S.236.20N Fonds Wetenschappelijk Onderzoek |
فهرسة مساهمة: | Keywords: Bovine mastitis; Cloxacillin; Endolysin; Mouse model; Penicillin; Streptococcus uberis |
المشرفين على المادة: | O6X5QGC2VB (Cloxacillin) EC 3.4.99.- (endolysin) EC 3.4.- (Endopeptidases) |
SCR Organism: | Streptococcus uberis |
تواريخ الأحداث: | Date Created: 20240111 Date Completed: 20240112 Latest Revision: 20240119 |
رمز التحديث: | 20240119 |
مُعرف محوري في PubMed: | PMC10781846 |
DOI: | 10.1007/s00253-023-12820-w |
PMID: | 38204128 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1432-0614 |
---|---|
DOI: | 10.1007/s00253-023-12820-w |