دورية أكاديمية
The influence of radiation-induced bystander effect in osteoblasts mediated by plasma-derived extracellular vesicles (EVs).
| العنوان: | The influence of radiation-induced bystander effect in osteoblasts mediated by plasma-derived extracellular vesicles (EVs). |
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| المؤلفون: | Du Y; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, China. Electronic address: cercocody@foxmail.com., Zhang Y; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China. Electronic address: 2020224035084@stu.scu.edu.cn., Luo W; Department of Stomatology, The First People's Hospital of Liangshan Yi Autonomous Prefecture, Sichuan province, China. Electronic address: lwqads@163.com., Gan F; Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, China. Electronic address: Agan94@foxmail.com., Yang M; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, China. Electronic address: yangmaodentist@163.com., Gong P; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, China. Electronic address: dentistgong@hotmail.com., Yao Y; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, China. Electronic address: yaoyang9999@126.com. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Feb 05; Vol. 695, pp. 149425. Date of Electronic Publication: 2023 Dec 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Bystander Effect* , Extracellular Vesicles*/metabolism, Humans ; Rats ; Animals ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Osteoblasts/metabolism |
| مستخلص: | Objectives: Head and neck tumor patients may develop post-radiotherapy diseases after radiotherapy treatment. And radiotherapy can elicit radiation-induced bystander effect, wherein extracellular vesicles (EVs) play a crucial role. For normal parts of the body that have not been directly irradiated, the effect of EVs on them needs to be further explored. This study aims to investigate the functions of plasma-derived EVs in regulating normal osteoblasts during radiation-induced bystander effects. Methods and Materials: Rat plasma-derived EVs were isolated and identified firstly, followed by an evaluation of their intracellular biological effects on normal osteoblasts in vitro. Transcriptome sequencing analysis and confirmations were performed to identify potential mechanisms. Results: Irradiated plasma-derived EVs were found to enhance osteoblast proliferation, migration, and cell cycle progression, concurrently suppressing the expression of osteogenesis-related genes and proteins. Furthermore, these EVs attenuated the expression of osteogenesis and oxidative stress resistance related genes, while upregulating the PI3K-AKT pathway and intracellular reactive oxygen species in osteoblasts. Conclusions: Irradiated plasma-derived EVs could alter the biological effects in osteoblasts, which is closely associated with the levels of GPX1 and the PI3K-AKT signaling pathway. This suggests that plasma-derived EVs serve as a crucial factor contributing to radiation-induced bystander effect in osteoblasts. Competing Interests: Declaration of competing interest All authors declare no conflict of interests, and this work is original research that has not been published previously, nor is under consideration for publication elsewhere, in whole or in part. All authors have read, agreed upon and approved the manuscript that is enclosed. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone metabolism; Extracellular vesicles; Osteogenesis; Radiation-induced bystander effect; Therapeutic resistance to radiation; Tumor radiotherapy |
| المشرفين على المادة: | EC 2.7.1.- (Phosphatidylinositol 3-Kinases) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) |
| تواريخ الأحداث: | Date Created: 20240111 Date Completed: 20240122 Latest Revision: 20240122 |
| رمز التحديث: | 20240122 |
| DOI: | 10.1016/j.bbrc.2023.149425 |
| PMID: | 38211533 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2023.149425 |