دورية أكاديمية
Comparative analysis of a novel N-butanol-prepared antigen vs thermo-resistant and sonicated antigens for human leptospirosis detection.
| العنوان: | Comparative analysis of a novel N-butanol-prepared antigen vs thermo-resistant and sonicated antigens for human leptospirosis detection. |
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| المؤلفون: | Blanco RM; Instituto Adolfo Lutz, Centro de Bacteriologia, Laboratório de Leptospirose, 01246-000 São Paulo, SP, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Departamento de Doenças Infecciosas e Parasitárias, 01246-903 São Paulo, SP, Brazil., Lima EDS; Instituto Adolfo Lutz, Centro de Bacteriologia, Laboratório de Leptospirose, 01246-000 São Paulo, SP, Brazil., de Haro GN; Universidade de São Paulo, Faculdade de Medicina, Departamento de Doenças Infecciosas e Parasitárias, 01246-903 São Paulo, SP, Brazil., Kamikawa CM; Instituto Adolfo Lutz, Centro de Imunologia, Laboratório de Imunodiagnóstico das Micoses, 01246-000 São Paulo, SP, Brazil., Blanco RM; Associação Nova Escola 05422-001 São Paulo, SP, Brazil., Vincentini AP; Instituto Adolfo Lutz, Centro de Imunologia, Laboratório de Imunodiagnóstico das Micoses, 01246-000 São Paulo, SP, Brazil., Romero EC; Instituto Adolfo Lutz, Centro de Bacteriologia, Laboratório de Leptospirose, 01246-000 São Paulo, SP, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Departamento de Doenças Infecciosas e Parasitárias, 01246-903 São Paulo, SP, Brazil. |
| المصدر: | Letters in applied microbiology [Lett Appl Microbiol] 2024 Jan 02; Vol. 77 (1). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Internet ISSN: 1472-765X (Electronic) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985- |
| مواضيع طبية MeSH: | Leptospirosis*/diagnosis , Leptospira*, Animals ; Cattle ; Humans ; 1-Butanol ; Butanols ; Antigens, Bacterial ; Enzyme-Linked Immunosorbent Assay/methods ; Antibodies, Bacterial ; Immunoglobulin M ; Sensitivity and Specificity |
| مستخلص: | The diagnosis of human leptospirosis is mainly based on serological assays. Since the extraction by N-butanol has only been studied as an antigen for the diagnosis of cattle leptospirosis, this study aimed to investigate the feasibility of the N-butanol preparation for the diagnosis of human leptospirosis and compare it with sonicated and thermo-resistant antigens in IgM dot-blot test. Paired serum samples from 147 laboratory-confirmed leptospirosis cases were tested. The control group consisted of 148 serum samples from healthy individuals and nonleptospirosis cases. N-butanol antigens from serovar Copenhageni (ButC3) and serovar Patoc (ButP3) showed reactivity with antileptospiral antibodies from patients with confirmed leptospirosis. In the acute phase, sensitivities of IgM dot-blot assay with ButC3 and ButP3 antigens were 47.6% and 51.0%, respectively. In the convalescent phase, sensitivities were 95.9% (ButC3) and 93.2% (ButP3), and no significant differences were observed among the IgM dot-blot tests with other antigens. The specificity of the IgM dot-blot test with ButC3 antigen was good (92.6%), but with ButP3 (83.1%), it was significantly lower than with the other tests. The IgM dot-blot test described in this study is simple to perform and presents reliable visual results. Antigens prepared by N-butanol proved to be valuable diagnostic markers of leptospirosis. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.) |
| فهرسة مساهمة: | Keywords: N-butanol; dot-blot; leptospiral antigens; leptospirosis; serodiagnosis |
| المشرفين على المادة: | 8PJ61P6TS3 (1-Butanol) 0 (Butanols) 0 (Antigens, Bacterial) 0 (Antibodies, Bacterial) 0 (Immunoglobulin M) |
| تواريخ الأحداث: | Date Created: 20240112 Date Completed: 20240202 Latest Revision: 20240202 |
| رمز التحديث: | 20240202 |
| DOI: | 10.1093/lambio/ovae004 |
| PMID: | 38216452 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1472-765X |
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| DOI: | 10.1093/lambio/ovae004 |