دورية أكاديمية
Observation of a single protein by ultrafast X-ray diffraction.
| العنوان: | Observation of a single protein by ultrafast X-ray diffraction. |
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| المؤلفون: | Ekeberg T; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden., Assalauova D; Deutsches Electronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany., Bielecki J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Boll R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Daurer BJ; Diamond Light Source, Harwell Science & Innovation Campus, Didcot, OX11 0DE, UK., Eichacker LA; University of Stavanger, Centre Organelle Research, Richard-Johnsensgate 4, 4021, Stavanger, Norway., Franken LE; Leibniz Institute for Experimental Virology (HPI), Centre for Structural Systems Biology, Notkestraße 85, 22607, Hamburg, Germany., Galli DE; Dipartimento di Fisica 'Aldo Pontremoli', Università degli Studi di Milano, via Celoria 16, 20133, Milano, Italy., Gelisio L; Deutsches Electronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany., Gumprecht L; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany., Gunn LH; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden.; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA., Hajdu J; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden., Hartmann R; PNSensor GmbH, Otto-Hahn-Ring 6, D-81739, München, Germany., Hasse D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden., Ignatenko A; Deutsches Electronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany., Koliyadu J; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691, Stockholm, Sweden., Kulyk O; ELI Beamlines/IoP Institute of Physics AS CR, v.v.i., Na Slovance 2, 182 21, Prague 8, Czech Republic., Kurta R; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Kuster M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Lugmayr W; Multi-User CryoEM Facility, Centre for Structural Systems Biology, Notkestr.85, 22607, Hamburg, Germany.; University Medical Center Hamburg-Eppendorf (UKE), Martinistrasse 52, 20246, Hamburg, Germany., Lübke J; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany.; The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Mancuso AP; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia., Mazza T; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Nettelblad C; Division of Scientific Computing, Science for Life Laboratory, Department of Information Technology, Uppsala University, Box 337, SE-75105, Uppsala, Sweden., Ovcharenko Y; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Rivas DE; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Rose M; Deutsches Electronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany., Samanta AK; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany., Schmidt P; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Sobolev E; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; European Molecular Biology Laboratory, c/o DESY, Notkestrasse 85, 22607, Hamburg, Germany., Timneanu N; Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120, Uppsala, Sweden., Usenko S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Westphal D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden., Wollweber T; The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761, Hamburg, Germany.; Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761, Hamburg, Germany., Worbs L; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Xavier PL; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany.; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761, Hamburg, Germany., Yousef H; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Ayyer K; The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761, Hamburg, Germany.; Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761, Hamburg, Germany., Chapman HN; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany.; The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Sellberg JA; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691, Stockholm, Sweden., Seuring C; Multi-User CryoEM Facility, Centre for Structural Systems Biology, Notkestr.85, 22607, Hamburg, Germany.; Department of Chemistry, Universität Hamburg, 20146, Hamburg, Germany., Vartanyants IA; Deutsches Electronen-Synchrotron DESY, Notkestrasse 85, 22607, Hamburg, Germany., Küpper J; Center for Free-Electron Laser Science, DESY, 22607, Hamburg, Germany.; The Hamburg Center for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany., Meyer M; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany., Maia FRNC; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124, Uppsala, Sweden. filipe.maia@icm.uu.se.; NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA. filipe.maia@icm.uu.se. |
| المصدر: | Light, science & applications [Light Sci Appl] 2024 Jan 12; Vol. 13 (1), pp. 15. Date of Electronic Publication: 2024 Jan 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Nature Country of Publication: England NLM ID: 101610753 Publication Model: Electronic Cited Medium: Internet ISSN: 2047-7538 (Electronic) Linking ISSN: 20477538 NLM ISO Abbreviation: Light Sci Appl Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Springer Nature, [2012]- |
| مستخلص: | The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to theory, the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier, and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably explodes. This was first demonstrated on biological samples a decade ago on the giant mimivirus. Since then, a large collaboration has been pushing the limit of the smallest sample that can be imaged. The ability to capture snapshots on the timescale of atomic vibrations, while keeping the sample at room temperature, may allow probing the entire conformational phase space of macromolecules. Here we show the first observation of an X-ray diffraction pattern from a single protein, that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays, and demonstrate that the concept of diffraction before destruction extends to single proteins. From the pattern, it is possible to determine the approximate orientation of the protein. Our experiment demonstrates the feasibility of ultrafast imaging of single proteins, opening the way to single-molecule time-resolved studies on the femtosecond timescale. (© 2024. The Author(s).) |
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| تواريخ الأحداث: | Date Created: 20240112 Latest Revision: 20240115 |
| رمز التحديث: | 20240115 |
| مُعرف محوري في PubMed: | PMC10786860 |
| DOI: | 10.1038/s41377-023-01352-7 |
| PMID: | 38216563 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2047-7538 |
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| DOI: | 10.1038/s41377-023-01352-7 |