دورية أكاديمية
Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion.
| العنوان: | Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion. |
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| المؤلفون: | Mandal T; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India., Biswas A; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India.; Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore, 117411, Singapore., Ghosh T; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India., Manikandan S; NORDITA, KTH Royal Institute of Technology and Stockholm University, Roslagstullsbacken 23, 10691, Stockholm, Sweden., Kundu A; Department of Physical Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India.; Experimental Physics I, Universität Bayreuth, Universitätsstraße 30, 95447, Bayreuth, Germany., Banerjee A; Department of Physical Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India., Mitra D; NORDITA, KTH Royal Institute of Technology and Stockholm University, Roslagstullsbacken 23, 10691, Stockholm, Sweden., Sinha B; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India. bidisha.sinha@iiserkol.ac.in. |
| المصدر: | Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2024 Jan 13; Vol. 81 (1), pp. 43. Date of Electronic Publication: 2024 Jan 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Switzerland NLM ID: 9705402 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-9071 (Electronic) Linking ISSN: 1420682X NLM ISO Abbreviation: Cell Mol Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basel : Springer Original Publication: Basel ; Boston : Birkhauser, c1997- |
| مواضيع طبية MeSH: | Clathrin*/metabolism , Coated Pits, Cell-Membrane*/metabolism, Humans ; HeLa Cells ; Endocytosis/physiology ; Cholesterol/metabolism ; Adenosine Triphosphate/metabolism ; Cell Membrane/metabolism |
| مستخلص: | Adherent cells ensure membrane homeostasis during de-adhesion by various mechanisms, including endocytosis. Although mechano-chemical feedbacks involved in this process have been studied, the step-by-step build-up and resolution of the mechanical changes by endocytosis are poorly understood. To investigate this, we studied the de-adhesion of HeLa cells using a combination of interference reflection microscopy, optical trapping and fluorescence experiments. We found that de-adhesion enhanced membrane height fluctuations of the basal membrane in the presence of an intact cortex. A reduction in the tether force was also noted at the apical side. However, membrane fluctuations reveal phases of an initial drop in effective tension followed by saturation. The area fractions of early (Rab5-labelled) and recycling (Rab4-labelled) endosomes, as well as transferrin-labelled pits close to the basal plasma membrane, also transiently increased. On blocking dynamin-dependent scission of endocytic pits, the regulation of fluctuations was not blocked, but knocking down AP2-dependent pit formation stopped the tension recovery. Interestingly, the regulation could not be suppressed by ATP or cholesterol depletion individually but was arrested by depleting both. The data strongly supports Clathrin and AP2-dependent pit-formation to be central to the reduction in fluctuations confirmed by super-resolution microscopy. Furthermore, we propose that cholesterol-dependent pits spontaneously regulate tension under ATP-depleted conditions. (© 2024. The Author(s).) |
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| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: Excess area regulation; Membrane homeostasis; Tension propagation |
| المشرفين على المادة: | 0 (Clathrin) 97C5T2UQ7J (Cholesterol) 8L70Q75FXE (Adenosine Triphosphate) |
| تواريخ الأحداث: | Date Created: 20240113 Date Completed: 20240115 Latest Revision: 20240210 |
| رمز التحديث: | 20240210 |
| مُعرف محوري في PubMed: | PMC10787898 |
| DOI: | 10.1007/s00018-023-05072-4 |
| PMID: | 38217571 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1420-9071 |
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| DOI: | 10.1007/s00018-023-05072-4 |