دورية أكاديمية
أعرض في EDS

Detection of oxalyl-CoA decarboxylase (oxc) and formyl-CoA transferase (frc) genes in novel probiotic isolates capable of oxalate degradation in vitro.

التفاصيل البيبلوغرافية
العنوان: Detection of oxalyl-CoA decarboxylase (oxc) and formyl-CoA transferase (frc) genes in novel probiotic isolates capable of oxalate degradation in vitro.
المؤلفون: Youssef HIA; Microbiology Department, Faculty of Science, Ain Shams University, El-Khalyfa El-Mamoun st. Abbasya, Cairo, 11566, Egypt. hoba83@sci.asu.edu.eg.
المصدر: Folia microbiologica [Folia Microbiol (Praha)] 2024 Apr; Vol. 69 (2), pp. 423-432. Date of Electronic Publication: 2024 Jan 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 0376757 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1874-9356 (Electronic) Linking ISSN: 00155632 NLM ISO Abbreviation: Folia Microbiol (Praha) Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Springer
Original Publication: Praha, Academia Scientiarum Bohemoslovenica.
مواضيع طبية MeSH: Carboxy-Lyases*/genetics , Probiotics* , Acyl Coenzyme A*, Humans ; Coenzyme A-Transferases/genetics ; Coenzyme A-Transferases/metabolism ; Oxalates/metabolism
مستخلص: Oxalate degradation is one of lactic acid bacteria's desirable activities. It is achieved by two enzymes, formyl coenzyme A transferase (frc) and oxalyl coenzyme A decarboxylase (oxc). The current study aimed to screen 15 locally isolated lactic acid bacteria to select those with the highest oxalate degradation ability. It also aimed to amplify the genes involved in degradation. MRS broth supplemented with 20 mM sodium oxalate was used to culture the tested isolates for 72 h. This was followed by an enzymatic assay to detect remaining oxalate. All isolates showed oxalate degradation activity to variable degrees. Five isolates demonstrated high oxalate degradation, 78 to 88%. To investigate the oxalate-degradation potential of the selected isolates, they have been further tested for the presence of genes that encode for enzymes involved in oxalate catabolism, formyl coenzyme A transferase (frc) and oxalyl coenzyme A decarboxylase (oxc). Three strains showed bands with the specific OXC and FRC forward and reverse primers designated as (SA-5, 9 and 37). Species-level identification revealed Loigolactobacillus bifermentans, Lacticaseibacillus paracasei, and Lactiplantibacillus plantarum. Preliminary results revealed that the tested probiotic strains harbored both oxc and frc whose products are putatively involved in oxalate catabolism. The probiotic potential of the selected strains was evaluated, and they showed high survival rates to both simulated gastric and intestinal fluids and variable degrees of antagonism against the tested Gram-positive and negative pathogens and were sensitive to clarithromycin but resistant to both metronidazole and ceftazidime. Finally, these strains could be exploited as an innovative approach to establish oxalate homeostasis in humans and prevent kidney stone formation.
(© 2024. The Author(s).)
References: Vet Microbiol. 2004 Jul 14;101(3):161-6. (PMID: 15223120)
Appl Environ Microbiol. 2002 Aug;68(8):3841-7. (PMID: 12147479)
Appl Environ Microbiol. 1999 Nov;65(11):4949-56. (PMID: 10543808)
J Appl Microbiol. 2014 Jun;116(6):1657-67. (PMID: 24655128)
Adv Nutr. 2011 May;2(3):254-60. (PMID: 22332057)
J Food Sci. 2014 Mar;79(3):M384-90. (PMID: 24471378)
Probiotics Antimicrob Proteins. 2012 Dec;4(4):217-26. (PMID: 26782181)
J Biol Chem. 2004 Aug 20;279(34):36003-12. (PMID: 15213226)
Vet Res Commun. 2023 Sep;47(3):1285-1302. (PMID: 36749534)
PLoS One. 2021 Nov 19;16(11):e0260116. (PMID: 34797858)
Urology. 2018 Mar;113:253.e1-253.e7. (PMID: 29198849)
Biomed Res Int. 2013;2013:280432. (PMID: 23971028)
Biomed J. 2017 Oct;40(5):270-283. (PMID: 29179882)
Am J Clin Pathol. 1966 Apr;45(4):493-6. (PMID: 5325707)
Appl Environ Microbiol. 2014 Mar;80(5):1595-601. (PMID: 24362432)
Int J Biol Macromol. 2021 Jul 31;183:423-434. (PMID: 33932415)
Urology. 2015 Mar;85(3):517-21. (PMID: 25733259)
PLoS One. 2019 Sep 23;14(9):e0222393. (PMID: 31545840)
Ann Transl Med. 2017 Jan;5(2):36. (PMID: 28217701)
Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3906-12. (PMID: 15671160)
Front Microbiol. 2016 Aug 30;7:1371. (PMID: 27625647)
Am J Vet Res. 2015 Sep;76(9):801-6. (PMID: 26309108)
J Biol Chem. 2005 Dec 16;280(50):41645-54. (PMID: 16216870)
Appl Environ Microbiol. 2008 Aug;74(15):4610-25. (PMID: 18539810)
Appl Environ Microbiol. 2010 Aug;76(16):5609-20. (PMID: 20601517)
J Clin Microbiol. 2021 Nov 18;59(12):e0021321. (PMID: 34550809)
Cells. 2022 Jan 14;11(2):. (PMID: 35053400)
J Am Soc Nephrol. 2008 Jun;19(6):1197-203. (PMID: 18322162)
J Am Soc Nephrol. 2004 Jun;15(6):1567-73. (PMID: 15153567)
Biochemistry. 2006 Aug 29;45(34):10344-50. (PMID: 16922510)
N Engl J Med. 2010 Sep 2;363(10):954-63. (PMID: 20818905)
FEMS Microbiol Ecol. 2007 Jul;61(1):110-20. (PMID: 17506826)
Urol Res. 2010 Jun;38(3):169-78. (PMID: 20224931)
فهرسة مساهمة: Keywords: Degradation; Formyl-CoA transferase; LAB; Oxalate; Oxalyl-CoA decarboxylase; Probiotic
المشرفين على المادة: 0 (oxalyl-coenzyme A)
EC 2.8.3.- (formyl-coenzyme A transferase)
EC 2.8.3.- (Coenzyme A-Transferases)
EC 4.1.1.8 (oxalyl CoA decarboxylase)
EC 4.1.1.- (Carboxy-Lyases)
0 (Oxalates)
0 (Acyl Coenzyme A)
تواريخ الأحداث: Date Created: 20240113 Date Completed: 20240411 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC11003902
DOI: 10.1007/s12223-024-01128-5
PMID: 38217756
قاعدة البيانات: MEDLINE
الوصف
تدمد:1874-9356
DOI:10.1007/s12223-024-01128-5