دورية أكاديمية
أعرض في EDS

Comparison of bovine viral diarrhea virus detection methods: Results of an international proficiency trial.

التفاصيل البيبلوغرافية
العنوان: Comparison of bovine viral diarrhea virus detection methods: Results of an international proficiency trial.
المؤلفون: Wernike K; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Insel Riems, Germany. Electronic address: kerstin.wernike@fli.de., Beer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Insel Riems, Germany.
المصدر: Veterinary microbiology [Vet Microbiol] 2024 Mar; Vol. 290, pp. 109985. Date of Electronic Publication: 2024 Jan 11.
نوع المنشور: Clinical Trial, Veterinary; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier Scientific Pub. Co.
مواضيع طبية MeSH: Border disease virus* , Bovine Virus Diarrhea-Mucosal Disease* , Cattle Diseases* , Diarrhea Virus 1, Bovine Viral* , Diarrhea Viruses, Bovine Viral* , Pestivirus* , Sheep Diseases*, Animals ; Cattle ; Antibodies, Viral ; Diarrhea/veterinary ; Enzyme-Linked Immunosorbent Assay/veterinary ; Enzyme-Linked Immunosorbent Assay/methods ; Sheep ; Sheep, Domestic
مستخلص: Bovine viral diarrhea virus (BVDV), one of the most important infectious cattle diseases globally, is being combated in multiple countries. The main source for virus transmission within herds and especially to unaffected cattle farms are life-long persistently infected (PI), immunotolerant animals. Therefore, the early identification of PI calves is a major pillar of disease control programs. In addition, rapid and reliable virus identification is necessary to confirm the causative agent in acute clinical cases. Here, we initiated an international interlaboratory proficiency trial in order to evaluate BVDV detection methods. Four ear notch samples and four sera were provided to the participating veterinary diagnostic laboratories (n = 40). Two of the ear notches and two sera contained BVDV and two ear notches and one serum were negative for pestiviruses. The remaining serum was positive for the ovine border disease virus (BDV). The sample panel was analyzed by an E RNS -based ELISA for antigen detection, diverse real-time RT-PCR (RT-qPCR) assays and/or virus isolation. Occasionally, additional typing of the virus strains was performed by sequencing or specific antibody staining of the obtained cell culture isolates. While the antigen ELISA allowed reliable BVDV diagnostics, infectious virus could be isolated only in just under half of the attempts (43.33%). RT-qPCR enabled the sensitive detection of pestiviruses, though an impact of the extraction method on the resulting quantification cycle values was observed. In general, subsequent typing of the detected virus strains is required to differentiate BVDV from BDV infections. In conclusion, for BVDV identification in clinical cases or in the context of disease control, RT-qPCR methods or E RNS antigen ELISAs should be preferentially used.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Antigen ELISA; Bovine viral diarrhea; Diagnostics; Pestivirus; Proficiency testing; RT-qPCR
المشرفين على المادة: 0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20240114 Date Completed: 20240221 Latest Revision: 20240304
رمز التحديث: 20240304
DOI: 10.1016/j.vetmic.2024.109985
PMID: 38219410
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2542
DOI:10.1016/j.vetmic.2024.109985