دورية أكاديمية
Mutations in starch biosynthesis genes affect chloroplast development in wheat pericarp.
| العنوان: | Mutations in starch biosynthesis genes affect chloroplast development in wheat pericarp. |
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| المؤلفون: | Botticella E; Department of Agriculture and Forest Science, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy; Institute of Sciences of Food Production (ISPA), National Research Council (CNR), via Provinciale Lecce-Monteroni, 73100 Lecce, Italy., Testone G; Institute for Biological Systems, National Research Council (CNR), Via Salaria, km 29.300, Monterotondo, 00015, Rome, Italy. Electronic address: giulio.testone@cnr.it., Buffagni V; Department of Agriculture and Forest Science, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy; Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy., Palombieri S; Department of Agriculture and Forest Science, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy., Taddei AR; Center of Large Equipments, Section of Electron Microscopy, University of Tuscia, Largo dell'Università, 01100 Viterbo, Italy., Lafiandra D; Department of Agriculture and Forest Science, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy., Lucini L; Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Via Emilia Parmense, 84, 29122 Piacenza, Italy., Giannino D; Institute for Biological Systems, National Research Council (CNR), Via Salaria, km 29.300, Monterotondo, 00015, Rome, Italy., Sestili F; Department of Agriculture and Forest Science, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy. Electronic address: francescosestili@unitus.it. |
| المصدر: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Feb; Vol. 207, pp. 108354. Date of Electronic Publication: 2024 Jan 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Paris : Gauthier-Villars, c1987- |
| مواضيع طبية MeSH: | Amylose*/metabolism , Triticum*/metabolism, Plant Proteins/genetics ; Plant Proteins/metabolism ; Starch/metabolism ; Mutation/genetics ; Chloroplasts/genetics ; Chloroplasts/metabolism |
| مستخلص: | Starch bioengineering in cereals has produced a plethora of genotypes with new nutritional and technological functionalities. Modulation of amylose content from 0 to 100% was inversely correlated with starch digestibility and promoted a lower glycemic index in food products. In wheat, starch mutants have been reported to exhibit various side effects, mainly related to the seed phenotype. However, little is known about the impact of altered amylose content and starch structure on plant metabolism. Here, three bread wheat starch mutant lines with extreme phenotypes in starch branching and amylose content were used to study plant responses to starch structural changes. Omics profiling of gene expression and metabolic patterns supported changes, confirmed by ultrastructural analysis in the chloroplast of the immature seeds. In detail, the identification of differentially expressed genes belonging to functional categories related to photosynthesis, chloroplast and thylakoid (e.g. CURT1), the alteration in the accumulation of photosynthesis-related compounds, and the chloroplast alterations (aberrant shape, grana stacking alteration, and increased number of plastoglobules) suggested that the modification of starch structure greatly affects starch turnover in the chloroplast, triggering oxidative stress (ROS accumulation) and premature tissue senescence. In conclusion, this study highlighted a correlation between starch structure and chloroplast functionality in the wheat kernel. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chloroplast; Seed omics; Side effects; Starch mutants; Wheat |
| المشرفين على المادة: | 9005-82-7 (Amylose) 0 (Plant Proteins) 9005-25-8 (Starch) |
| تواريخ الأحداث: | Date Created: 20240114 Date Completed: 20240318 Latest Revision: 20240318 |
| رمز التحديث: | 20240318 |
| DOI: | 10.1016/j.plaphy.2024.108354 |
| PMID: | 38219425 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2690 |
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| DOI: | 10.1016/j.plaphy.2024.108354 |