دورية أكاديمية
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Metal-organic framework-based multienzyme cascade bioreactor for sensitive detection of methyl parathion.

التفاصيل البيبلوغرافية
العنوان: Metal-organic framework-based multienzyme cascade bioreactor for sensitive detection of methyl parathion.
المؤلفون: Chen D; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Wang L; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Wei J; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Jiao T; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Chen Q; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Oyama M; Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8520, Japan., Chen Q; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China., Chen X; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China., Chen X; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, China. Electronic address: xmchen@jmu.edu.cn.
المصدر: Food chemistry [Food Chem] 2024 Jun 01; Vol. 442, pp. 138389. Date of Electronic Publication: 2024 Jan 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
مواضيع طبية MeSH: Methyl Parathion* , Metal-Organic Frameworks* , Metal Nanoparticles*, Acetylcholinesterase ; Acetylcholine ; Gold ; Limit of Detection
مستخلص: In this study, a cascade nanobioreactor was developed for the highly sensitive detection of methyl parathion (MP) in food samples. The simultaneous encapsulation of acetylcholinesterase (AChE) and choline oxidase (CHO) in a zeolitic imidazole ester backbone (ZIF-8) effectively improved the stability and cascade catalytic efficiency of the enzymes. In addition, glutathione-stabilized gold nanoclusters (GSH-AuNCs) were encapsulated in ZIF-8 by ligand self-assembly, conferring excellent fluorescence properties. Acetylcholine (ATCh) is catalyzed by a cascade of AChE/CHO@ZIF-8 as well as Fe(II) to generate hydroxyl radicals (·OH) with strong oxidizing properties. The ·OH radicals then oxidize Au(0) in GSH-AuNCs@ZIF-8 to Au(I), resulting in fluorescence quenching. MP, as an inhibitor of AChE, hinders the cascade reaction and thus restores the fluorescence emission, enabling its quantitative detection. The limit of detection of the constructed nanobioreactor for MP was 0.23 µg/L. This MOF-based cascade nanobioreactor has great potential for the detection of trace hazards.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Cascade response; Fluorescence; Methyl parathion; Nanobioreactor; ZIF-8
المشرفين على المادة: 41BCL2O91D (Methyl Parathion)
0 (Metal-Organic Frameworks)
EC 3.1.1.7 (Acetylcholinesterase)
N9YNS0M02X (Acetylcholine)
7440-57-5 (Gold)
تواريخ الأحداث: Date Created: 20240114 Date Completed: 20240214 Latest Revision: 20240214
رمز التحديث: 20240214
DOI: 10.1016/j.foodchem.2024.138389
PMID: 38219569
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-7072
DOI:10.1016/j.foodchem.2024.138389