دورية أكاديمية
Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery.
| العنوان: | Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery. |
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| المؤلفون: | Sedovy MW; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA.; Translational Biology, Medicine, and Health Graduate Program, Virginia Tech, Blacksburg, Virginia, USA., Leng X; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA., Iqbal F; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA.; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA., Renton MC; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA., Leaf M; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA.; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA., Roberts K; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA., Malek A; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA., Arnold WS; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA., Wyatt DA; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA., Choate CW; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA., Rowe JF; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA., Baker JW; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA., Johnstone SR; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA.; Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA., Joseph M; The Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Roanoke, Virginia, USA.; Division of Cardiothoracic Surgery, Department of Surgery, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, USA.; Carilion Cardiothoracic Surgery, Cardiovascular Institute, Carilion Clinic, Roanoke, Virginia, USA. |
| المصدر: | Journal of vascular research [J Vasc Res] 2024; Vol. 61 (2), pp. 68-76. Date of Electronic Publication: 2024 Jan 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: S. Karger Country of Publication: Switzerland NLM ID: 9206092 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0135 (Electronic) Linking ISSN: 10181172 NLM ISO Abbreviation: J Vasc Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel ; New York : S. Karger, c1992- |
| مواضيع طبية MeSH: | Saphenous Vein*/pathology , Endothelial Cells*, Humans ; NF-E2-Related Factor 2 ; Endothelium, Vascular/pathology ; Coronary Artery Bypass/adverse effects |
| مستخلص: | Introduction: While multiple factors influence coronary artery bypass graft (CABG) success rates, preserving saphenous vein endothelium during surgery may improve patency. Standard preparations include saphenous vein preparation in heparinized saline (saline) which can result in endothelial loss and damage. Here, we investigated the impact of preparing saphenous graft vessels in heparinized patient blood (blood) versus saline. Methods: Saphenous vein tissues from a total of 23 patients undergoing CABG were split into 2 groups (1) saline and (2) heparinized patient blood. Excess tissue was fixed for analysis immediately following surgery. Level of endothelial coverage, oxidative stress marker 4-hydroxynonenal (4HNE), and oxidative stress protective marker nuclear factor erythroid 2-related factor 2 (NRF2) were evaluated. Results: In saline patient veins, histological analysis revealed a limited luminal layer, suggesting a loss of endothelial cells (ECs). Immunofluorescent staining of EC markers vascular endothelial cadherin (VE-cadherin) and endothelial nitric oxide identified a significant improvement in EC coverage in the blood versus saline groups. Although both treatment groups expressed 4HNE to similar levels, EC blood samples expressed higher levels of NRF2. Conclusion: Our data indicate that use of heparinized patient blood helps preserve the endothelium and promotes vein graft health. This has the potential to improve long-term outcomes in patients. (© 2024 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Coronary artery bypass graft; Endothelial cells; Saphenous vein; Smooth muscle cell |
| المشرفين على المادة: | 0 (NF-E2-Related Factor 2) |
| تواريخ الأحداث: | Date Created: 20240114 Date Completed: 20240408 Latest Revision: 20240408 |
| رمز التحديث: | 20240408 |
| DOI: | 10.1159/000535843 |
| PMID: | 38219725 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1423-0135 |
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| DOI: | 10.1159/000535843 |