دورية أكاديمية
Investigation of the antioxidant effect of Chrysin in an experimental cataract model created in chick embryos.
| العنوان: | Investigation of the antioxidant effect of Chrysin in an experimental cataract model created in chick embryos. |
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| المؤلفون: | Kurt GA; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Afyonkarahisar, Turkey., Ertekin T; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Afyonkarahisar, Turkey., Atay E; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Afyonkarahisar, Turkey., Bilir A; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Afyonkarahisar, Turkey., Koca HB; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Medical Biochemistry, Afyonkarahisar, Turkey., Aslan E; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Histology and Embryology, Afyonkarahisar, Turkey., Sarıtaş A; Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Anatomy, Afyonkarahisar, Turkey. |
| المصدر: | Molecular vision [Mol Vis] 2023 Nov 05; Vol. 29, pp. 245-255. Date of Electronic Publication: 2023 Nov 05 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Molecular Vision Country of Publication: United States NLM ID: 9605351 Publication Model: eCollection Cited Medium: Internet ISSN: 1090-0535 (Electronic) Linking ISSN: 10900535 NLM ISO Abbreviation: Mol Vis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Atlanta Ga : Molecular Vision, 1995- |
| مواضيع طبية MeSH: | Cataract*/chemically induced , Cataract*/drug therapy , Cataract*/pathology , Lens, Crystalline*/pathology, Animals ; Chick Embryo ; Antioxidants/pharmacology ; Flavonoids/pharmacology ; Glutathione |
| مستخلص: | Purpose: Cataract, which occurs as a result of lens opacification, is one of the most common causes of vision loss. In the literature, deterioration of the antioxidant system due to the increase in reactive oxygen species and oxidant levels is shown among the causes of cataract formation. The aim of this study was to investigate the antioxidant effect of chrysin on steroid-induced cataract development in an experimental chick embryo model using morphological, histological and biochemical parameters. Methods: Within the scope of the study, 150 specific pathogen free (SPF) fertilized eggs were used. Eggs were divided into 6 groups as control (group 1), corn oil (group 2), hydrocortisone hemisuccinate sodium (HC) (group 3), low dose chrysin (group 4), medium dose chrysin (group 5) and high dose chrysin (group 6). On the 15th day of incubation, Chrysin and HC were applicated to the air sac of the eggs with Hamilton and/or insulin injector. On day 17, the chick embryos were removed from the eggs and the bulbus oculi of the embryos were dissected. Lenses of 9 embryos were used for morpholigical cataract grading in each group, lens of 8 embryos for biochemical analysis and intact eyes of 7 embryos for histological evaluation (TUNEL method). Results: No opacity was observed in any of the lenses in Group 1 and 2. Cataract was observed in all lenses in Group 3. The mean opacity grades in group 3 were statistically significantly higher when compared to group 1 and 2 (p<0.05). The difference between group 6 and group 3 was statistically significant (p<0.05). GSH and TAS levels in the lenses were statistically significantly decreased compared to the control group due to HC application (p<0.05). It was determined that the decreased GSH and TAS levels in the lenses increased in relation to the Chrysin application doses. The increased levels of MDA, TOS, caspase 3 and caspase 9 in the HC group decreased significantly depending to the chrysin doses (p<0.05). In addition, while the rate of apoptotic cells determined by the TUNEL method was statistically significantly higher in the HC administered group than in the control group (p<0.05), it was statistically significantly decreased in the chrysin-administered groups, in relation to the dose of chrysin (p<0.05). Conclusions: We think that anti-cataract effect of crhysin may be due to the antioxidant and antiapoptotic properties of chrysin. However, more research is needed to clarify the anti-cataract effects of chrysin. (Copyright © 2023 Molecular Vision.) |
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| المشرفين على المادة: | 0 (Antioxidants) 3CN01F5ZJ5 (chrysin) 0 (Flavonoids) GAN16C9B8O (Glutathione) |
| تواريخ الأحداث: | Date Created: 20240115 Date Completed: 20240116 Latest Revision: 20240313 |
| رمز التحديث: | 20240314 |
| مُعرف محوري في PubMed: | PMC10784222 |
| PMID: | 38222446 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-0535 |
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