التفاصيل البيبلوغرافية
| العنوان: |
Cell-Free Expressed Membraneless Organelles Inhibit Translation in Synthetic Cells. |
| المؤلفون: |
Robinson AO; Department of Genetics, Cell Biology and Development, University of Minnesota, 420 SE Washington Ave., Minneapolis, Minnesota 55455, United States., Lee J; Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, Pennsylvania 16802, United States., Cameron A; Department of Genetics, Cell Biology and Development, University of Minnesota, 420 SE Washington Ave., Minneapolis, Minnesota 55455, United States., Keating CD; Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, Pennsylvania 16802, United States., Adamala KP; Department of Genetics, Cell Biology and Development, University of Minnesota, 420 SE Washington Ave., Minneapolis, Minnesota 55455, United States. |
| المصدر: |
ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2024 Feb 12; Vol. 10 (2), pp. 773-781. Date of Electronic Publication: 2024 Jan 16. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, DC : American Chemical Society, [2015]- |
| مواضيع طبية MeSH: |
Artificial Cells*, Animals ; Biomolecular Condensates ; Cytoplasm/metabolism ; Organelles/metabolism ; Proteins/metabolism ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism |
| مستخلص: |
Compartments within living cells create specialized microenvironments, allowing multiple reactions to be carried out simultaneously and efficiently. While some organelles are bound by a lipid bilayer, others are formed by liquid-liquid phase separation such as P-granules and nucleoli. Synthetic minimal cells are widely used to study many natural processes, including organelle formation. In this work, synthetic cells expressing artificial membrane-less organelles that inhibit translation are described. RGG-GFP-RGG, a phase-separating protein derived from Caenorhabditis elegans P-granules, is expressed by cell-free transcription and translation, forming artificial membraneless organelles that can sequester RNA and reduce protein expression in synthetic cells. The introduction of artificial membrane-less organelles creates complex microenvironments within the synthetic cell cytoplasm and functions as a tool to inhibit protein expression in synthetic cells. The engineering of compartments within synthetic cells furthers the understanding of the evolution and function of natural organelles and facilitates the creation of more complex and multifaceted synthetic lifelike systems. |
| التعليقات: |
Update of: bioRxiv. 2023 Apr 03:2023.04.03.535479. doi: 10.1101/2023.04.03.535479. (PMID: 37066403) |
| فهرسة مساهمة: |
Keywords: cell-free expression; coacervates; membraneless organelles; synthetic biology; synthetic cells; translation |
| المشرفين على المادة: |
0 (Proteins) |
| تواريخ الأحداث: |
Date Created: 20240116 Date Completed: 20240214 Latest Revision: 20240612 |
| رمز التحديث: |
20240613 |
| DOI: |
10.1021/acsbiomaterials.3c01052 |
| PMID: |
38226971 |
| قاعدة البيانات: |
MEDLINE |
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