دورية أكاديمية
أعرض في EDS

Transcriptional profile of ribosome-associated quality control components and their associated phenotypes in mammalian cells.

التفاصيل البيبلوغرافية
العنوان: Transcriptional profile of ribosome-associated quality control components and their associated phenotypes in mammalian cells.
المؤلفون: Dos Santos OAL; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil., Carneiro RL; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil., Requião RD; Departamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil., Ribeiro-Alves M; Fundação Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro, 21040-900, Brazil., Domitrovic T; Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil., Palhano FL; Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil. palhano@bioqmed.ufrj.br.
المصدر: Scientific reports [Sci Rep] 2024 Jan 16; Vol. 14 (1), pp. 1439. Date of Electronic Publication: 2024 Jan 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae Proteins*/metabolism, Animals ; Humans ; Protein Biosynthesis ; Ubiquitination ; Ribosomes/genetics ; Ribosomes/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Ubiquitin-Protein Ligases/metabolism ; Mammals/metabolism
مستخلص: During protein synthesis, organisms detect translation defects that induce ribosome stalling and result in protein aggregation. The Ribosome-associated Quality Control (RQC) complex, comprising TCF25, LTN1, and NEMF, is responsible for identifying incomplete protein products from unproductive translation events, targeting them for degradation. Although RQC disruption causes adverse effects on vertebrate neurons, data regarding mRNA/protein expression and regulation across tissues are lacking. Employing high-throughput methods, we analyzed public datasets to explore RQC gene expression and phenotypes. Our findings revealed widespread expression of RQC components in human tissues; however, silencing of RQC yielded only mild negative effects on cell growth. Notably, TCF25 exhibited elevated mRNA levels that were not reflected in the protein content. We experimentally demonstrated that this disparity arose from post-translational protein degradation by the proteasome. Additionally, we observed that cellular aging marginally influenced RQC expression, leading to reduced mRNA levels in specific tissues. Our results suggest the necessity of RQC expression in all mammalian tissues. Nevertheless, when RQC falters, alternative mechanisms seem to compensate, ensuring cell survival under nonstress conditions.
(© 2024. The Author(s).)
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المشرفين على المادة: 0 (Saccharomyces cerevisiae Proteins)
0 (RNA, Messenger)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
تواريخ الأحداث: Date Created: 20240116 Date Completed: 20240118 Latest Revision: 20240119
رمز التحديث: 20240120
مُعرف محوري في PubMed: PMC10792078
DOI: 10.1038/s41598-023-50811-z
PMID: 38228636
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-50811-z