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An octameric PqiC toroid stabilises the outer-membrane interaction of the PqiABC transport system.

التفاصيل البيبلوغرافية
العنوان: An octameric PqiC toroid stabilises the outer-membrane interaction of the PqiABC transport system.
المؤلفون: Cooper BF; Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, Oxford, UK., Ratkevičiūtė G; Department of Biochemistry, University of Oxford, OX1 3QU, Oxford, UK., Clifton LA; ISIS Pulsed Neutron & Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory Harwell Oxford Campus, OX11 OQX, Didcot, UK., Johnston H; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Holyfield R; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Hardy DJ; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Caulton SG; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Chatterton W; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Sridhar P; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Wotherspoon P; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Hughes GW; Institute of Cancer and Genomic Sciences, University of Birmingham, B15 2TT, Birmingham, UK., Hall SC; ISIS Pulsed Neutron & Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory Harwell Oxford Campus, OX11 OQX, Didcot, UK., Lovering AL; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK., Knowles TJ; School of Biosciences, University of Birmingham, B15 2TT, Birmingham, UK. t.j.knowles@bham.ac.uk.
المصدر: EMBO reports [EMBO Rep] 2024 Jan; Vol. 25 (1), pp. 82-101. Date of Electronic Publication: 2024 Jan 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 100963049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-3178 (Electronic) Linking ISSN: 1469221X NLM ISO Abbreviation: EMBO Rep Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : [London] : Nature Publishing Group
Original Publication: Oxford, UK : Published for EMBO by Oxford University Press, 2000-
مواضيع طبية MeSH: Escherichia coli*/genetics , Escherichia coli*/metabolism , Escherichia coli Proteins*/chemistry, Membrane Proteins/metabolism ; Biological Transport ; Lipoproteins/metabolism
مستخلص: The E. coli Paraquat Inducible (Pqi) Pathway is a putative Gram-negative phospholipid transport system. The pathway comprises three components: an integral inner membrane protein (PqiA), a periplasmic spanning MCE family protein (PqiB) and an outer membrane lipoprotein (PqiC). Interactions between all complex components, including stoichiometry, remain uncharacterised; nevertheless, once assembled into their quaternary complex, the trio of Pqi proteins are anticipated to provide a continuous channel between the inner and outer membranes of diderms. Here, we present X-ray structures of both the native and a truncated, soluble construct of the PqiC lipoprotein, providing insight into its biological assembly, and utilise neutron reflectometry to characterise the nature of the PqiB-PqiC-membrane interaction. Finally, we employ phenotypic complementation assays to probe specific PqiC residues, which imply the interaction between PqiB and PqiC is less intimate than previously anticipated.
(© 2024. The Author(s).)
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معلومات مُعتمدة: BB/M01116X/1 UKRI | Biotechnology and Biological Sciences Research Council (BBSRC); BB/S017283/1 UKRI | Biotechnology and Biological Sciences Research Council (BBSRC); RB2300004 UKRI | STFC | ISIS Neutron and Muon Source (The ISIS Neutron and Muon Source); RB2220703 UKRI | STFC | ISIS Neutron and Muon Source (The ISIS Neutron and Muon Source); mx26803 Diamond Light Source
فهرسة مساهمة: Keywords: Envelope Spanning; Gram Negative Cell Envelope; Lipid Transport; Outer Membrane Biogenesis; Pqi
المشرفين على المادة: 0 (Escherichia coli Proteins)
0 (Membrane Proteins)
0 (Lipoproteins)
تواريخ الأحداث: Date Created: 20240116 Date Completed: 20240124 Latest Revision: 20240229
رمز التحديث: 20240229
مُعرف محوري في PubMed: PMC10897342
DOI: 10.1038/s44319-023-00014-4
PMID: 38228789
قاعدة البيانات: MEDLINE
الوصف
تدمد:1469-3178
DOI:10.1038/s44319-023-00014-4