دورية أكاديمية
Improved Specificity of Glutamate Decarboxylase 65 Autoantibody Measurement Using Luciferase-Based Immunoprecipitation System Assays.
| العنوان: | Improved Specificity of Glutamate Decarboxylase 65 Autoantibody Measurement Using Luciferase-Based Immunoprecipitation System Assays. |
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| المؤلفون: | Wyatt RC; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Grace SL; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Brigatti C; San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy., Marzinotto I; San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy., Gillard BT; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Shoemark DK; School of Biochemistry, University of Bristol, Bristol, U.K., Chandler K; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Achenbach P; Institute of Diabetes Research, Helmholtz Munich, German Center for Environmental Health, Munich, Germany.; Technical University of Munich, School of Medicine, Klinikum rechts der Isar, Forschergruppe Diabetes, Munich, Germany., Piemonti L; Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Milan, Italy., Long AE; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Gillespie KM; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K., Lampasona V; Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Milan, Italy., Williams AJK; Diabetes and Metabolism Unit, Translational Health Sciences, University of Bristol, Bristol, U.K. |
| مؤلفون مشاركون: | BOX Study Group |
| المصدر: | Diabetes [Diabetes] 2024 Apr 01; Vol. 73 (4), pp. 565-571. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Diabetes Association Country of Publication: United States NLM ID: 0372763 Publication Model: Print Cited Medium: Internet ISSN: 1939-327X (Electronic) Linking ISSN: 00121797 NLM ISO Abbreviation: Diabetes Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Alexandria, VA : American Diabetes Association Original Publication: [New York, American Diabetes Association] |
| مواضيع طبية MeSH: | Diabetes Mellitus, Type 1*, Humans ; Glutamate Decarboxylase ; Sensitivity and Specificity ; Autoantibodies ; Luciferases/genetics ; Immunoprecipitation |
| مستخلص: | Autoantibodies to glutamate decarboxylase (GADA) are widely used in the prediction and classification of type 1 diabetes. GADA radiobinding assays (RBAs) using N-terminally truncated antigens offer improved specificity, but radioisotopes limit the high-throughput potential for population screening. Luciferase-based immunoprecipitation system (LIPS) assays are sensitive and specific alternatives to RBAs with the potential to improve risk stratification. The performance of assays using the Nanoluc luciferase (Nluc)-conjugated GAD65 constructs, Nluc-GAD65(96-585) and full length Nluc-GAD65(1-585), were evaluated in 434 well-characterized serum samples from patients with recent-onset type 1 diabetes and first-degree relatives. Nonradioactive, high-throughput LIPS assays are quicker and require less serum than RBAs. Of 171 relatives previously tested single autoantibody positive for autoantibodies to full-length GAD65 by RBA but had not progressed to diabetes, fewer retested positive by LIPS using either truncated (n = 72) or full-length (n = 111) antigen. The Nluc-GAD65(96-585) truncation demonstrated the highest specificity in LIPS assays overall, but in contrast to RBA, N-terminus truncations did not result in a significant increase in disease-specificity compared with the full-length antigen. This suggests that binding of nonspecific antibodies is affected by the conformational changes resulting from addition of the Nluc antigen. Nluc-GAD65(96-585) LIPS assays offer low-blood-volume, high-specificity GADA tests for screening and diagnostics. (© 2024 by the American Diabetes Association.) |
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| معلومات مُعتمدة: | 14/0004472 United Kingdom DUK_ Diabetes UK |
| فهرسة مساهمة: | Investigator: I Wilson; R Aitken; I Kelland; C Megson; C Ballav; A Dutta; M Russell-Taylor; R Besser; J Bursell; S Chandran; S Patel; A Smith; M Kenchaiah; G Margabanthu; F Kavvoura; C Yaliwal |
| المشرفين على المادة: | EC 4.1.1.15 (Glutamate Decarboxylase) 0 (Autoantibodies) EC 1.13.12.- (Luciferases) |
| تواريخ الأحداث: | Date Created: 20240117 Date Completed: 20240322 Latest Revision: 20240324 |
| رمز التحديث: | 20240324 |
| مُعرف محوري في PubMed: | PMC10958581 |
| DOI: | 10.2337/db23-0550 |
| PMID: | 38232306 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1939-327X |
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| DOI: | 10.2337/db23-0550 |