دورية أكاديمية
A novel pectate lyase with high specific activity from Bacillus sp. B58-2: Gene cloning, heterologous expression and use in ramie degumming.
| العنوان: | A novel pectate lyase with high specific activity from Bacillus sp. B58-2: Gene cloning, heterologous expression and use in ramie degumming. |
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| المؤلفون: | Liu S; School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, Guangxi, PR China., Qin Y; National Key Laboratory of Non-food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning 530007, PR China., Wang Q; National Key Laboratory of Non-food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning 530007, PR China., Zhang J; School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, Guangxi, PR China., Zhou J; School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, Guangxi, PR China., He B; School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, Guangxi, PR China., Liang X; School of Light Industry and Food Engineering, Guangxi University, Nanning 530000, Guangxi, PR China. Electronic address: 19890011@gxu.edu.cn., Xian L; National Key Laboratory of Non-food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning 530007, PR China. Electronic address: liangxianpublish@163.com., Wu J; National Key Laboratory of Non-food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning 530007, PR China. Electronic address: xxwjh@sina.com. |
| المصدر: | Enzyme and microbial technology [Enzyme Microb Technol] 2024 Apr; Vol. 175, pp. 110395. Date of Electronic Publication: 2024 Jan 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Elsevier Original Publication: [Guildford, Eng.] IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Bacillus* , Boehmeria*/genetics, Calcium/metabolism ; Cloning, Molecular ; Polysaccharide-Lyases/metabolism ; Weight Loss ; Hydrogen-Ion Concentration |
| مستخلص: | Pectinase plays a crucial role in ramie degumming. A gene encoding a putative pectate lyase from Bacillus sp. strain B58-2 was cloned and heterologously expressed in Escherichia coli. The amplified gene BvelPL1 encoded a mature protein of 400 amino acids. BvelPL1 shared the highest amino acid sequence identity (78.75%) with the enzymatically characterized pectate lyase Pel from Bacillus subtilis strain RCK (GenBank: AFH66771.1). The purified recombinant enzyme rBvelPL1-Ec exhibited a maximum specific activity of 2433.26 U/mg at pH 8.5 and 50 °C towards polygalacturonic acid. This specific activity was higher than that of most reported pectate lyases. Remarkably, the enzymatic activity of rBvelPL1-Ec increased by 23.28 times in the presence of 0.4 mM calcium ion. The effect of calcium ion on promoting the enzymatic activity of rBvelPL1-Ec was greater than that for all reported pectate lyases. After degumming with rBvelPL1-Ec, a weight loss of 21.27 ± 1.17% of circled ramie fibers was obtained, and the surfaces of the ramie fibers became smoother. Moreover, a weight loss of 30.47 ± 0.46% was obtained through enzymatic treated and subsequent NaOH treated circled ramie fibers. The excellent performance in degumming suggests that rBvelPL1-Ec may serve as a promising biocatalyst in the textile industry. Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bacillus; Ca(2+) promotion effect; Gene cloning and expression; Pectate lyase; Ramie degumming; Specific enzyme activity |
| المشرفين على المادة: | EC 4.2.2.2 (pectate lyase) SY7Q814VUP (Calcium) EC 4.2.2.- (Polysaccharide-Lyases) |
| تواريخ الأحداث: | Date Created: 20240118 Date Completed: 20240226 Latest Revision: 20240226 |
| رمز التحديث: | 20240226 |
| DOI: | 10.1016/j.enzmictec.2024.110395 |
| PMID: | 38237242 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0909 |
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| DOI: | 10.1016/j.enzmictec.2024.110395 |