دورية أكاديمية
Halloween genes are expressed with a circadian rhythm during development in prothoracic glands of the insect RHODNIUS PROLIXUS.
| العنوان: | Halloween genes are expressed with a circadian rhythm during development in prothoracic glands of the insect RHODNIUS PROLIXUS. |
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| المؤلفون: | Vafopoulou X; Department of Biology, York University, Toronto M3J 1P3, Canada., Steel CGH; Department of Biology, York University, Toronto M3J 1P3, Canada. Electronic address: csteel@yorku.ca. |
| المصدر: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2024 Apr; Vol. 290, pp. 111588. Date of Electronic Publication: 2024 Jan 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9806096 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4332 (Electronic) Linking ISSN: 10956433 NLM ISO Abbreviation: Comp Biochem Physiol A Mol Integr Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, : Elsevier Science, c1998- |
| مواضيع طبية MeSH: | Rhodnius*/genetics , Rhodnius*/metabolism , Insect Hormones*/genetics , Insect Hormones*/metabolism, Animals ; Ecdysteroids/metabolism ; Circadian Rhythm/physiology ; Larva/metabolism |
| مستخلص: | We analyse the developmental and circadian profiles of expression of the genes responsible for ecdysteroidogenesis (Halloween genes) in the PGs of Rhodnius prolixus throughout larval-adult development. Extensive use of in vitro techniques enabled multiple different parameters to be measured in individual PGs. Expression of disembodied and spook closely paralleled the ecdysteroid synthesis of the same PGs, and the ecdysteroid titre in vivo, but with functionally significant exceptions. Various tissues other than PGs expressed one, both or neither genes. Both gonads express both genes in pharate adults (larvae close to ecdysis). Both genes were expressed at low, but significant, levels in UF Rhodnius, raising questions concerning how developmental arrest is maintained in UF animals. IHC confirmed the subcellular localisation of the coded proteins. Gene knockdown suppressed transcription of both genes and ecdysteroid synthesis, with spook apparently regulating the downstream gene disembodied. Transcription of both genes occurred with a daily rhythm (with peaks at night) that was confirmed to be under circadian control using aperiodic conditions. The complex behaviour of the rhythm in LL implied two anatomically distinct oscillators regulate this transcription rhythm. First, the circadian clock in the PGs and second, the circadian rhythm of of Rhodnius PTTH which is released rhythmically from the brain under control of the circadian clock therein, both of which were described previously. We conclude ecdysteroidogenesis in Rhodnius PGs employs a similar pathway as other insects, but its control is complex, involving mechanisms both within and outside the PGs. Competing Interests: Declaration of competing interest There is no conflict of interest with anyof the entities listed. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Circadian transcription; Ecdysteroid synthesis; Gene knockdown; Moulting; Prothoracicotropic hormone |
| المشرفين على المادة: | 0 (Ecdysteroids) 0 (Insect Hormones) |
| تواريخ الأحداث: | Date Created: 20240119 Date Completed: 20240214 Latest Revision: 20240515 |
| رمز التحديث: | 20240516 |
| DOI: | 10.1016/j.cbpa.2024.111588 |
| PMID: | 38242349 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1531-4332 |
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| DOI: | 10.1016/j.cbpa.2024.111588 |