دورية أكاديمية
Standardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom.
| العنوان: | Standardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom. |
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| المؤلفون: | Oliveira Orsi R; College of Veterinary Medicine and Animal Sciences (FMVZ), São Paulo State University (UNESP), Botucatu, Brazil.; Graduate Program in Animal Science, College of Veterinary Medicine, and Animal Sciences (FMVZ), São Paulo State University (UNESP), Botucatu, Brazil., Zaluski R; Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Brazil., de Barros LC; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, Brazil., Barraviera B; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, Brazil.; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, Brazil.; Graduate Program in Clinical Research, Botucatu Medical School (FMB) and CEVAP, São Paulo State University (UNESP), Botucatu, Brazil., Pimenta DC; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, Brazil.; Laboratory of Biochemistry and Biophysics, Butantan Institute (BI), São Paulo, Brazil., Ferreira Junior RS; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, Brazil.; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, Brazil.; Graduate Program in Clinical Research, Botucatu Medical School (FMB) and CEVAP, São Paulo State University (UNESP), Botucatu, Brazil. |
| المصدر: | Journal of toxicology and environmental health. Part B, Critical reviews [J Toxicol Environ Health B Crit Rev] 2024 Feb 17; Vol. 27 (2), pp. 73-90. Date of Electronic Publication: 2024 Jan 26. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 9802627 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-6950 (Electronic) Linking ISSN: 10937404 NLM ISO Abbreviation: J Toxicol Environ Health B Crit Rev Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Washington, DC : Taylor & Francis, c1998- |
| مواضيع طبية MeSH: | Insect Bites and Stings*/drug therapy , Bee Venoms*/analysis , Bee Venoms*/chemistry, Bees ; Humans ; Animals ; Antivenins/therapeutic use ; Melitten/analysis ; Melitten/chemistry ; Phospholipases A2 ; Antigens |
| مستخلص: | Africanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the last 5 years with a total of 303 deaths. To treat such massive attacks, Brazilian researchers developed the first specific antivenom against Africanized honey bee sting exposure. This unique product, the first of its kind in the world, has been safely tested in 20 patients during a Phase 2 clinical trial. To develop the antivenom, a standardized process was undertaken to extract primary venom antigens from the Africanized bees for immunization of serum-producing horses. This process involved extracting, purifying, fractionating, characterizing, and identifying the venom (apitoxin) employing mass spectrometry to generate standardized antigen for hyperimmunization of horses using the major toxins (melittin and its isoforms and phospholipase A2). The current guide describes standardization of the entire production chain of venom antigens in compliance with good manufacturing practices (GMP) required by regulatory agencies. Emphasis is placed upon the welfare of bees and horses during this process, as well as the development of a new biopharmaceutical to ultimately save lives. |
| فهرسة مساهمة: | Keywords: Africanized honeybee; Apis mellifera; good manufacturing practices; hyperimmunization; standard operating procedures |
| المشرفين على المادة: | 0 (Antivenins) 0 (Bee Venoms) 20449-79-0 (Melitten) EC 3.1.1.4 (Phospholipases A2) 0 (Antigens) |
| تواريخ الأحداث: | Date Created: 20240122 Date Completed: 20240129 Latest Revision: 20240129 |
| رمز التحديث: | 20240129 |
| DOI: | 10.1080/10937404.2023.2300786 |
| PMID: | 38247328 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1521-6950 |
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| DOI: | 10.1080/10937404.2023.2300786 |