دورية أكاديمية
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Chitosan-Polyethylene Glycol Inspired Polyelectrolyte Complex Hydrogel Templates Favoring NEO-Tissue Formation for Cardiac Tissue Engineering.

التفاصيل البيبلوغرافية
العنوان: Chitosan-Polyethylene Glycol Inspired Polyelectrolyte Complex Hydrogel Templates Favoring NEO-Tissue Formation for Cardiac Tissue Engineering.
المؤلفون: Keklikian A; Department of Translational Research, College of Osteopathic Medicine of the Pacific and Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA., de Barros NR; Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, CA 90064, USA., Rashad A; Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, CA 90064, USA., Chen Y; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Tan J; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Sheng R; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Sun D; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Liu H; Department of Bioengineering, University of California, Riverside, CA 92521, USA., Thankam FG; Department of Translational Research, College of Osteopathic Medicine of the Pacific and Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, CA 91766, USA.
المصدر: Gels (Basel, Switzerland) [Gels] 2024 Jan 08; Vol. 10 (1). Date of Electronic Publication: 2024 Jan 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101696925 Publication Model: Electronic Cited Medium: Internet ISSN: 2310-2861 (Electronic) Linking ISSN: 23102861 NLM ISO Abbreviation: Gels Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2015]-
مستخلص: Neo-tissue formation and host tissue regeneration determine the success of cardiac tissue engineering where functional hydrogel scaffolds act as cardiac (extracellular matrix) ECM mimic. Translationally, the hydrogel templates promoting neo-cardiac tissue formation are currently limited; however, they are highly demanding in cardiac tissue engineering. The current study focused on the development of a panel of four chitosan-based polyelectrolyte hydrogels as cardiac scaffolds facilitating neo-cardiac tissue formation to promote cardiac regeneration. Chitosan-PEG (CP), gelatin-chitosan-PEG (GCP), hyaluronic acid-chitosan-PEG (HACP), and combined CP (CoCP) polyelectrolyte hydrogels were engineered by solvent casting and assessed for physiochemical, thermal, electrical, biodegradable, mechanical, and biological properties. The CP, GCP, HACP, and CoCP hydrogels exhibited excellent porosity (4.24 ± 0.18, 13.089 ± 1.13, 12.53 ± 1.30 and 15.88 ± 1.10 for CP, GCP, HACP and CoCP, respectively), water profile, mechanical strength, and amphiphilicity suitable for cardiac tissue engineering. The hydrogels were hemocompatible as evident from the negligible hemolysis and RBC aggregation and increased adsorption of plasma albumin. The hydrogels were cytocompatible as evident from the increased viability by MTT (>94% for all the four hydrogels) assay and direct contact assay. Also, the hydrogels supported the adhesion, growth, spreading, and proliferation of H9c2 cells as unveiled by rhodamine staining. The hydrogels promoted neo-tissue formation that was proven using rat and swine myocardial tissue explant culture. Compared to GCP and CoCP, CP and HACP were superior owing to the cell viability, hemocompatibility, and conductance, resulting in the highest degree of cytoskeletal organization and neo-tissue formation. The physiochemical and biological performance of these hydrogels supported neo-cardiac tissue formation. Overall, the CP, GCP, HACP, and CoCP hydrogel systems promise novel translational opportunities in regenerative cardiology.
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فهرسة مساهمة: Keywords: cardiac tissue engineering; chitosan; neo-tissue formation; polyelectrolyte hydrogels; regenerative cardiology
تواريخ الأحداث: Date Created: 20240122 Latest Revision: 20240128
رمز التحديث: 20240128
مُعرف محوري في PubMed: PMC10815274
DOI: 10.3390/gels10010046
PMID: 38247769
قاعدة البيانات: MEDLINE
الوصف
تدمد:2310-2861
DOI:10.3390/gels10010046