دورية أكاديمية
Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation.
| العنوان: | Mechanisms of SARS-CoV-2 Inactivation Using UVC Laser Radiation. |
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| المؤلفون: | Devitt G; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Johnson PB; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Hanrahan N; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Lane SIR; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Vidale MC; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Sheth B; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Allen JD; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Humbert MV; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Sir Henry Wellcome Laboratories, University Hospital Southampton, Southampton SO16 6YD, United Kingdom.; University of Cambridge, MRC Toxicology Unit, Cambridge, CB2 1QR, United Kingdom., Spalluto CM; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Sir Henry Wellcome Laboratories, University Hospital Southampton, Southampton SO16 6YD, United Kingdom.; Southampton NIHR Biomedical Research Centre, Southampton General Hospital, Southampton SO16 6YD, United Kingdom., Hervé RC; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Staples K; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Sir Henry Wellcome Laboratories, University Hospital Southampton, Southampton SO16 6YD, United Kingdom.; Wessex Investigational Sciences Hub, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.; Southampton NIHR Biomedical Research Centre, Southampton General Hospital, Southampton SO16 6YD, United Kingdom., West JJ; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Cancer Sciences, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, United Kingdom., Forster R; M Squared Lasers, Limited, 1 K Campus, West of Scotland Science Park, Glasgow, G20 0SP, United Kingdom., Divecha N; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., McCormick CJ; Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Sir Henry Wellcome Laboratories, University Hospital Southampton, Southampton SO16 6YD, United Kingdom., Crispin M; School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom., Hempler N; M Squared Lasers, Limited, 1 K Campus, West of Scotland Science Park, Glasgow, G20 0SP, United Kingdom., Malcolm GPA; M Squared Lasers, Limited, 1 K Campus, West of Scotland Science Park, Glasgow, G20 0SP, United Kingdom., Mahajan S; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.; Department of Biotechnology, Inland Norway University of Applied Sciences, Holsetgata 22, N-2317 Hamar, Norway. |
| المصدر: | ACS photonics [ACS Photonics] 2023 Dec 26; Vol. 11 (1), pp. 42-52. Date of Electronic Publication: 2023 Dec 26 (Print Publication: 2024). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101634366 Publication Model: eCollection Cited Medium: Print ISSN: 2330-4022 (Print) Linking ISSN: 23304022 NLM ISO Abbreviation: ACS Photonics Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, [2014-] |
| مستخلص: | Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) has had a tremendous impact on humanity. Prevention of transmission by disinfection of surfaces and aerosols through a chemical-free method is highly desirable. Ultraviolet C (UVC) light is uniquely positioned to achieve inactivation of pathogens. We report the inactivation of SARS-CoV-2 virus by UVC radiation and explore its mechanisms. A dose of 50 mJ/cm 2 using a UVC laser at 266 nm achieved an inactivation efficiency of 99.89%, while infectious virions were undetectable at 75 mJ/cm 2 indicating >99.99% inactivation. Infection by SARS-CoV-2 involves viral entry mediated by the spike glycoprotein (S), and viral reproduction, reliant on translation of its genome. We demonstrate that UVC radiation damages ribonucleic acid (RNA) and provide in-depth characterization of UVC-induced damage of the S protein. We find that UVC severely impacts SARS-CoV- 2 spike protein's ability to bind human angiotensin-converting enzyme 2 (hACE2) and this correlates with loss of native protein conformation and aromatic amino acid integrity. This report has important implications for the design and development of rapid and effective disinfection systems against the SARS-CoV-2 virus and other pathogens. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.) |
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| تواريخ الأحداث: | Date Created: 20240122 Latest Revision: 20240123 |
| رمز التحديث: | 20240123 |
| مُعرف محوري في PubMed: | PMC10797618 |
| DOI: | 10.1021/acsphotonics.3c00828 |
| PMID: | 38249683 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2330-4022 |
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| DOI: | 10.1021/acsphotonics.3c00828 |