Optimised Fermentation Production of Radiolabelled Ochratoxin A by Aspergillus ochraceus with Maximum 14 C in the Pentaketide Moiety for Exploring Its Rat Renal Toxicology.

التفاصيل البيبلوغرافية
العنوان:	Optimised Fermentation Production of Radiolabelled Ochratoxin A by Aspergillus ochraceus with Maximum ¹⁴ C in the Pentaketide Moiety for Exploring Its Rat Renal Toxicology.
المؤلفون:	Mantle P; Centre for Environmental Policy, Imperial College London, London SW7 2AZ, UK.
المصدر:	Toxins [Toxins (Basel)] 2023 Dec 22; Vol. 16 (1). Date of Electronic Publication: 2023 Dec 22.
نوع المنشور:	Review; Journal Article
اللغة:	English
بيانات الدورية:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Basel : MDPI
مواضيع طبية MeSH:	Balkan Nephropathy* , Mycotoxins* , Ochratoxins*, Male ; Animals ; Rats ; Aspergillus ochraceus ; Fermentation
مستخلص:	In the context of the mysterious Balkan endemic nephropathy of the 1900s, and the discovery in the 1960s of the potent mycotoxin ochratoxin A, experimental research projects sought to explore any inter-relationship. Experimental lifetime administration of the toxin to male rats had revealed renal DNA adducts with the toxin, correlated with renal tumours, confirmation of which required molecular evidence. Consequently, production of ¹⁴ C-ochratoxin A of a high specific radioactivity was required, practical biosynthetic detail of which had not previously been published. A fermentation study of Aspergillus ochraceous was carried out during 2002 for a European project, to select for the production of high-quality ¹⁴ C-ochratoxin A, necessarily exploring for the maximum diversion of ¹⁴ C-sodium acetate into the pentaketide portion of mycotoxin. Experimentation necessarily had to optimise the competitive context of fungal growth dynamics and addition of the biosynthetic precursor in the early days of shaken-flask fermentation before adding the radiolabelled precursor. From optimal fermentation, 50 mg of the ¹⁴ C ochratoxin A was supplied within a European project for DNA adduct experimentation, but that proved negative as subsequently published. Experimental description of the radiolabelled ochratoxin A production was later made in a doctoral thesis, but is first publicised here. Further review of the literature reveals an explanation for the published failure to confirm rat DNA/ochratoxin A adduct formation, for which further experimentation is now recommended. Competing Interests: The authors declare no conflicts of interest.
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فهرسة مساهمة:	Keywords: DNA adducts; OTA carcinogenesis; optimised kinetics; radioactive biosynthesis; shaken culture; wheat substrate
المشرفين على المادة:	1779SX6LUY (ochratoxin A) 0 (Mycotoxins) 0 (Ochratoxins)
تواريخ الأحداث:	Date Created: 20240122 Date Completed: 20240123 Latest Revision: 20240227
رمز التحديث:	20240227
مُعرف محوري في PubMed:	PMC10820727
DOI:	10.3390/toxins16010008
PMID:	38251225
قاعدة البيانات:	MEDLINE

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تدمد:	2072-6651
DOI:	10.3390/toxins16010008