دورية أكاديمية
A gel-coated air-liquid-interface culture system with tunable substrate stiffness matching healthy and diseased lung tissues.
| العنوان: | A gel-coated air-liquid-interface culture system with tunable substrate stiffness matching healthy and diseased lung tissues. |
|---|---|
| المؤلفون: | He ZJ; Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States., Chu C; Soft Biomatter Laboratory, Department of Materials Science and Engineering, University of Virginia, Charlottesville, Virginia, United States., Dickson R; Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia, United States., Okuda K; Marsico Lung Institute/Cystic Fibrosis Research Center, University of North Carolina, Chapel Hill, North Carolina, United States., Cai LH; Soft Biomatter Laboratory, Department of Materials Science and Engineering, University of Virginia, Charlottesville, Virginia, United States.; Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia, United States.; Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States. |
| المصدر: | American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2024 Mar 01; Vol. 326 (3), pp. L292-L302. Date of Electronic Publication: 2024 Jan 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901229 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1504 (Electronic) Linking ISSN: 10400605 NLM ISO Abbreviation: Am J Physiol Lung Cell Mol Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1989- |
| مواضيع طبية MeSH: | Airway Remodeling* , Lung Diseases*, Humans ; Epithelial Cells ; Lung ; Hydrogels ; Cells, Cultured |
| مستخلص: | Since its invention in the late 1980s, the air-liquid-interface (ALI) culture system has been the standard in vitro model for studying human airway biology and pulmonary diseases. However, in a conventional ALI system, cells are cultured on a porous plastic membrane that is much stiffer than human airway tissues. Here, we develop a gel-ALI culture system by simply coating the plastic membrane with a thin layer of hydrogel with tunable stiffness matching that of healthy and fibrotic airway tissues. We determine the optimum gel thickness that does not impair the transport of nutrients and biomolecules essential to cell growth. We show that the gel-ALI system allows human bronchial epithelial cells (HBECs) to proliferate and differentiate into pseudostratified epithelium. Furthermore, we discover that HBECs migrate significantly faster on hydrogel substrates with stiffness matching that of fibrotic lung tissues, highlighting the importance of mechanical cues in human airway remodeling. The developed gel-ALI system provides a facile approach to studying the effects of mechanical cues in human airway biology and in modeling pulmonary diseases. NEW & NOTEWORTHY In a conventional ALI system, cells are cultured on a plastic membrane that is much stiffer than human airway tissues. We develop a gel-ALI system by coating the plastic membrane with a thin layer of hydrogel with tunable stiffness matching that of healthy and fibrotic airway tissues. We discover that human bronchial epithelial cells migrate significantly faster on hydrogel substrates with pathological stiffness, highlighting the importance of mechanical cues in human airway remodeling. |
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| معلومات مُعتمدة: | OKUDA20G0 Cystic Fibrosis Foundation (CFF); R01 HL163602 United States HL NHLBI NIH HHS; R01HL163602 Foundation for the National Institutes of Health (FNIH); CAREER DMR-1944625 National Science Foundation (NSF); P30 DK065988 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: air-liquid interface; cell differentiation; collective cell migration; human bronchial epithelia; substrate stiffness |
| المشرفين على المادة: | 0 (Hydrogels) |
| تواريخ الأحداث: | Date Created: 20240122 Date Completed: 20240228 Latest Revision: 20240728 |
| رمز التحديث: | 20240728 |
| مُعرف محوري في PubMed: | PMC11280679 |
| DOI: | 10.1152/ajplung.00153.2023 |
| PMID: | 38252871 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1504 |
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| DOI: | 10.1152/ajplung.00153.2023 |