دورية أكاديمية
Investigating the Effects of Sex Hormones on Macrophage Polarization.
| العنوان: | Investigating the Effects of Sex Hormones on Macrophage Polarization. |
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| المؤلفون: | Enright S; Thrombosis and Atherosclerosis Research Institute, 237 Barton Street E, Hamilton, ON L8L 2X2, Canada., Werstuck GH; Thrombosis and Atherosclerosis Research Institute, 237 Barton Street E, Hamilton, ON L8L 2X2, Canada.; Department of Medicine, McMaster University, 1280 Main St. W, Hamilton, ON L8S 4L8, Canada. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2024 Jan 12; Vol. 25 (2). Date of Electronic Publication: 2024 Jan 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Cardiovascular Diseases* , Atherosclerosis*, Mice ; Adult ; Animals ; Female ; Humans ; Male ; Mice, Inbred C57BL ; Gonadal Steroid Hormones ; Estradiol/pharmacology ; Testosterone/pharmacology ; Interleukin-1beta/genetics ; Macrophages ; Anti-Inflammatory Agents |
| مستخلص: | Sex differences in the development and progression of cardiovascular disease are well established, but the effects of sex hormones on macrophage polarization and pro-atherogenic functions are not well described. We hypothesize that sex hormones directly modulate macrophage polarization, and thereby regulate the progression of atherosclerosis. Bone marrow-derived monocytes from adult male and female C57BL/6 mice were differentiated into macrophages using macrophage colony-stimulating factor (20 ng/mL) and pre-treated with either 17β-estradiol (100 nM), testosterone (100 nM), or a vehicle control for 24 h. Macrophages were polarized into pro- or anti-inflammatory phenotypes and the effects of sex hormone supplementation on the gene expression of macrophage phenotypic markers were assessed using RT-qPCR. Inflammatory markers, including IL-1β, were quantified using an addressable laser bead immunoassay. A transwell migration assay was used to determine changes in macrophage migration. Sex differences were observed in macrophage polarization, inflammatory responses, and migration. Pre-treatment with 17β-estradiol significantly impaired the gene expression of inflammatory markers and the production of IL-1β in inflammatory macrophages. In anti-inflammatory macrophages, 17β-estradiol significantly upregulated the expression of anti-inflammatory markers and enhanced migration. Pre-treatment with testosterone enhanced anti-inflammatory mRNA expression and impaired the production of IL-1β. Our observations suggest a protective role of 17β-estradiol in atherogenesis that may contribute to the sexual dimorphisms in cardiovascular disease observed in human patients. |
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| معلومات مُعتمدة: | PJT-166092 Canada CAPMC CIHR |
| فهرسة مساهمة: | Keywords: 17β-estradiol; macrophage polarization; testosterone |
| المشرفين على المادة: | 0 (Gonadal Steroid Hormones) 4TI98Z838E (Estradiol) 3XMK78S47O (Testosterone) 0 (Interleukin-1beta) 0 (Anti-Inflammatory Agents) |
| تواريخ الأحداث: | Date Created: 20240123 Date Completed: 20240124 Latest Revision: 20240128 |
| رمز التحديث: | 20240128 |
| مُعرف محوري في PubMed: | PMC10816176 |
| DOI: | 10.3390/ijms25020951 |
| PMID: | 38256027 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms25020951 |