دورية أكاديمية
Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology.
| العنوان: | Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology. |
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| المؤلفون: | Xia P; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Ma X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Yan L; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Lian S; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Li X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Luo Y; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Chen Z; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Ji X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2024 Jan 14; Vol. 25 (2). Date of Electronic Publication: 2024 Jan 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Antibodies, Monoclonal* , S100A12 Protein*, Swine ; Animals ; Hybridomas ; Calgranulin A ; Calgranulin B ; Technology |
| مستخلص: | S100A8, S100A9, and S100A12 proteins are important members of the S100 protein family, act primarily as congenital immunomodulators, and are closely related to the occurrence of infectious diseases. There have been few reports on the functional properties of S100A8, S100A9, and S100A12 proteins in swine, but it is certain that porcine S100A8, S100A9, and S100A12 proteins are highly expressed in diseased swine. To address the current lack of reliable and timely detection tools for these three proteins, we generated monoclonal antibodies specific to the porcine S100A8, S100A9, and S100A12 proteins using hybridoma technology. The results of serum sample testing showed that the above monoclonal antibodies specifically recognize the proteins S100A8, S100A9, and S100A12 in the serum and were able to evaluate the content change of these proteins during the infection process. This provides the basis for the use of porcine S100A8, S100A9, and S100A12 in the surveillance and diagnosis of swine diseases and laid a foundation for further understanding their roles in infection, immunity, and inflammation, as well as their potential applications in preventing or treating gastrointestinal tract or inflammatory diseases in swine. |
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| معلومات مُعتمدة: | 32072820, 31702242 National Science Foundation |
| فهرسة مساهمة: | Keywords: S100 proteins; hybridoma technology; monoclonal antibody; swine health |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (S100A12 Protein) 0 (Calgranulin A) 0 (Calgranulin B) |
| تواريخ الأحداث: | Date Created: 20240123 Date Completed: 20240124 Latest Revision: 20240128 |
| رمز التحديث: | 20240128 |
| مُعرف محوري في PubMed: | PMC10816078 |
| DOI: | 10.3390/ijms25021029 |
| PMID: | 38256103 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms25021029 |