دورية أكاديمية
Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology.
العنوان: | Generation and Application of Monoclonal Antibodies against Porcine S100A8, S100A9, and S100A12 Proteins Using Hybridoma Technology. |
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المؤلفون: | Xia P; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Ma X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Yan L; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Lian S; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Li X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Luo Y; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Chen Z; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China., Ji X; College of Veterinary Medicine (Institute of Comparative Medicine), Yangzhou University, Yangzhou 225009, China.; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.; Joint International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of China, Yangzhou University, Yangzhou 225009, China. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2024 Jan 14; Vol. 25 (2). Date of Electronic Publication: 2024 Jan 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | Antibodies, Monoclonal* , S100A12 Protein*, Swine ; Animals ; Hybridomas ; Calgranulin A ; Calgranulin B ; Technology |
مستخلص: | S100A8, S100A9, and S100A12 proteins are important members of the S100 protein family, act primarily as congenital immunomodulators, and are closely related to the occurrence of infectious diseases. There have been few reports on the functional properties of S100A8, S100A9, and S100A12 proteins in swine, but it is certain that porcine S100A8, S100A9, and S100A12 proteins are highly expressed in diseased swine. To address the current lack of reliable and timely detection tools for these three proteins, we generated monoclonal antibodies specific to the porcine S100A8, S100A9, and S100A12 proteins using hybridoma technology. The results of serum sample testing showed that the above monoclonal antibodies specifically recognize the proteins S100A8, S100A9, and S100A12 in the serum and were able to evaluate the content change of these proteins during the infection process. This provides the basis for the use of porcine S100A8, S100A9, and S100A12 in the surveillance and diagnosis of swine diseases and laid a foundation for further understanding their roles in infection, immunity, and inflammation, as well as their potential applications in preventing or treating gastrointestinal tract or inflammatory diseases in swine. |
References: | Dev Comp Immunol. 2011 Apr;35(4):490-500. (PMID: 21185856) Br J Nurs. 2015 Sep;24(16 Suppl 1):S4-13. (PMID: 26946646) Gastroenterology. 2011 May;140(6):1817-1826.e2. (PMID: 21530748) Pharmacol Res Perspect. 2019 Dec;7(6):e00535. (PMID: 31859459) Infect Immun. 2018 May 22;86(6):. (PMID: 29610259) Cell Res. 2010 Mar;20(3):314-31. (PMID: 19935772) Immunology. 2023 Nov 27;:. (PMID: 38013255) Biometals. 2023 Aug;36(4):817-828. (PMID: 36826733) Nat Rev Microbiol. 2022 Nov;20(11):657-670. (PMID: 35641670) Biochemistry. 2019 Apr 30;58(17):2269-2281. (PMID: 30957488) Mol Immunol. 2010 Apr;47(7-8):1601-7. (PMID: 20189650) Int J Mol Sci. 2014 Aug 08;15(8):13802-16. (PMID: 25110868) PLoS Pathog. 2014 Oct 16;10(10):e1004450. (PMID: 25330071) Nature. 1975 Aug 7;256(5517):495-7. (PMID: 1172191) World J Gastroenterol. 2014 Jan 14;20(2):363-75. (PMID: 24574706) Int J Mol Sci. 2022 Dec 31;24(1):. (PMID: 36614150) Mol Med Rep. 2015 Nov;12(5):7712-20. (PMID: 26459027) J Immunol Methods. 2010 Jan 31;352(1-2):13-22. (PMID: 19854197) Gut. 2007 Dec;56(12):1706-13. (PMID: 17675327) Biochemistry. 2020 Jul 7;59(26):2468-2478. (PMID: 32491853) J Basic Microbiol. 2015 Sep;55(9):1118-24. (PMID: 25847483) Inflamm Res. 2018 Oct;67(10):801-812. (PMID: 30083975) Am J Med. 2008 Dec;121(12):1099-106. (PMID: 19028207) ACS Appl Bio Mater. 2020 Aug 17;3(8):5395-5406. (PMID: 35021713) Biotechnol Lett. 2023 Jun;45(5-6):589-600. (PMID: 36971774) Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3841-6. (PMID: 23431180) Chembiochem. 2020 May 4;21(9):1372-1382. (PMID: 31821694) Infect Immun. 2015 Jul;83(7):2944-56. (PMID: 25964473) Adv Clin Chem. 2020;98:173-231. (PMID: 32564786) Korean J Intern Med. 2014 Jan;29(1):12-9. (PMID: 24574827) Nat Rev Cancer. 2015 Jun;15(6):361-70. (PMID: 25998715) Cytokine. 2019 Oct;122:154104. (PMID: 28756107) J Biol Chem. 2015 Jul 31;290(31):18991-8. (PMID: 26055713) Methods Mol Biol. 2022;2508:115-134. (PMID: 35737237) Biochim Biophys Acta Mol Cell Res. 2020 Jun;1867(6):118677. (PMID: 32057918) Vet Res. 2018 Feb 26;49(1):23. (PMID: 29482635) Nat Immunol. 2020 Mar;21(3):298-308. (PMID: 32066953) Exp Mol Med. 2013 Apr 26;45:e20. (PMID: 23619188) Int J Mol Sci. 2022 Dec 15;23(24):. (PMID: 36555597) Science. 2008 Feb 15;319(5865):962-5. (PMID: 18276893) ACS Chem Neurosci. 2016 Jan 20;7(1):34-9. (PMID: 26550994) |
معلومات مُعتمدة: | 32072820, 31702242 National Science Foundation |
فهرسة مساهمة: | Keywords: S100 proteins; hybridoma technology; monoclonal antibody; swine health |
المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (S100A12 Protein) 0 (Calgranulin A) 0 (Calgranulin B) |
تواريخ الأحداث: | Date Created: 20240123 Date Completed: 20240124 Latest Revision: 20240128 |
رمز التحديث: | 20240128 |
مُعرف محوري في PubMed: | PMC10816078 |
DOI: | 10.3390/ijms25021029 |
PMID: | 38256103 |
قاعدة البيانات: | MEDLINE |
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