دورية أكاديمية
أعرض في EDS

Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration.

التفاصيل البيبلوغرافية
العنوان: Gelatin-containing porous polycaprolactone PolyHIPEs as substrates for 3D breast cancer cell culture and vascular infiltration.
المؤلفون: Jackson CE; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom., Doyle I; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom., Khan H; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom., Williams SF; Department of Infection, Immunity and Cardiovascular Disease, Royal Hallamshire Hospital, The University of Sheffield, Sheffield, United Kingdom., Aldemir Dikici B; Department of Bioengineering, Izmir Institute of Technology, Urla, Türkiye., Barajas Ledesma E; Department of Chemistry, The University of Sheffield, Sheffield, United Kingdom., Bryant HE; School of Medicine and Population Health, University of Sheffield, Sheffield, United Kingdom., English WR; Norwich Medical School, University of East Anglia, Norwich, United Kingdom., Green NH; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom., Claeyssens F; The Kroto Research Institute, Materials Science and Engineering, University of Sheffield, Sheffield, United Kingdom.; Insigneo Institute for in Silico Medicine, The Pam Liversidge Building, University of Sheffield, Sheffield, United Kingdom.
المصدر: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Jan 08; Vol. 11, pp. 1321197. Date of Electronic Publication: 2024 Jan 08 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101632513 Publication Model: eCollection Cited Medium: Print ISSN: 2296-4185 (Print) Linking ISSN: 22964185 NLM ISO Abbreviation: Front Bioeng Biotechnol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Tumour survival and growth are reliant on angiogenesis, the formation of new blood vessels, to facilitate nutrient and waste exchange and, importantly, provide a route for metastasis from a primary to a secondary site. Whilst current models can ensure the transport and exchange of nutrients and waste via diffusion over distances greater than 200 μm, many lack sufficient vasculature capable of recapitulating the tumour microenvironment and, thus, metastasis. In this study, we utilise gelatin-containing polymerised high internal phase emulsion (polyHIPE) templated polycaprolactone-methacrylate (PCL-M) scaffolds to fabricate a composite material to support the 3D culture of MDA-MB-231 breast cancer cells and vascular ingrowth. Firstly, we investigated the effect of gelatin within the scaffolds on the mechanical and chemical properties using compression testing and FTIR spectroscopy, respectively. Initial in vitro assessment of cell metabolic activity and vascular endothelial growth factor expression demonstrated that gelatin-containing PCL-M polyHIPEs are capable of supporting 3D breast cancer cell growth. We then utilised the chick chorioallantoic membrane (CAM) assay to assess the angiogenic potential of cell-seeded gelatin-containing PCL-M polyHIPEs, and vascular ingrowth within cell-seeded, surfactant and gelatin-containing scaffolds was investigated via histological staining. Overall, our study proposes a promising composite material to fabricate a substrate to support the 3D culture of cancer cells and vascular ingrowth.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Jackson, Doyle, Khan, Williams, Aldemir Dikici, Barajas Ledesma, Bryant, English, Green and Claeyssens.)
References: J Invest Dermatol. 1983 Dec;81(6):485-8. (PMID: 6196418)
Macromol Biosci. 2011 May 12;11(5):618-27. (PMID: 21344647)
Adv Healthc Mater. 2023 Jul;12(17):e2203148. (PMID: 36802199)
Tissue Eng. 2001 Dec;7(6):679-89. (PMID: 11749726)
Cancer Res. 2017 May 15;77(10):2633-2646. (PMID: 28377452)
Vasc Health Risk Manag. 2006;2(3):213-9. (PMID: 17326328)
Stem Cell Res Ther. 2018 Mar 21;9(1):70. (PMID: 29562916)
Nature. 2004 Apr 1;428(6982):487-92. (PMID: 15057821)
Polymers (Basel). 2021 Mar 30;13(7):. (PMID: 33808492)
Biomater Sci. 2021 Oct 26;9(21):7297-7310. (PMID: 34617526)
Mater Sci Eng C Mater Biol Appl. 2020 Mar;108:110384. (PMID: 31924046)
Nat Protoc. 2006;1(1):85-91. (PMID: 17406216)
Microvasc Res. 2020 Sep;131:104026. (PMID: 32505611)
APL Bioeng. 2018 Apr 26;2(2):026103. (PMID: 31069300)
Biomed Opt Express. 2022 Apr 04;13(5):2616-2643. (PMID: 35774339)
Int J Mol Sci. 2021 Nov 11;22(22):. (PMID: 34830082)
Biomaterials. 2012 Apr;33(12):3401-10. (PMID: 22305104)
J Vasc Res. 1997 Nov-Dec;34(6):455-63. (PMID: 9425998)
Biomed Res Int. 2015;2015:865076. (PMID: 25883978)
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3190-3200. (PMID: 33405582)
Comput Biol Med. 2021 Sep;136:104647. (PMID: 34274599)
J Biomed Mater Res A. 2019 Oct;107(10):2123-2134. (PMID: 31094049)
Methods Protoc. 2018 May 31;1(2):. (PMID: 31164562)
Chem Soc Rev. 2009 Apr;38(4):1139-51. (PMID: 19421585)
Front Bioeng Biotechnol. 2020 Aug 12;8:875. (PMID: 32903473)
Biomaterials. 2007 Dec;28(36):5536-43. (PMID: 17889331)
Biomaterials. 2013 Oct;34(31):7754-65. (PMID: 23863451)
Mater Today Bio. 2019 Sep;4:100028. (PMID: 31853520)
Tissue Eng Part B Rev. 2020 Apr;26(2):164-180. (PMID: 31910095)
J Biomed Mater Res B Appl Biomater. 2012 Jul;100(5):1425-34. (PMID: 22279003)
Biomaterials. 2017 Jun;129:188-198. (PMID: 28343005)
Pediatr Res. 2008 May;63(5):487-91. (PMID: 18427292)
Bioengineering (Basel). 2023 Apr 26;10(5):. (PMID: 37237592)
Cancer Lett. 2018 Jan 1;412:88-98. (PMID: 29024811)
Pharmaceutics. 2022 May 31;14(6):. (PMID: 35745750)
Materials (Basel). 2019 Aug 20;12(16):. (PMID: 31434207)
ACS Appl Mater Interfaces. 2020 Mar 18;12(11):12510-12524. (PMID: 32100541)
Biomaterials. 2013 Aug;34(24):5915-25. (PMID: 23680364)
Int J Biomater. 2017;2017:8074890. (PMID: 29599800)
Cells. 2021 Feb 22;10(2):. (PMID: 33671534)
Biofabrication. 2019 Jun 12;11(3):035027. (PMID: 30991370)
Cell Mol Life Sci. 2020 May;77(9):1745-1770. (PMID: 31690961)
J Tissue Eng. 2020 Oct 20;11:2041731420942734. (PMID: 33194169)
Front Chem. 2023 Aug 23;11:1236944. (PMID: 37681209)
Phys Med Biol. 2020 Dec 16;65(23):. (PMID: 32998112)
Trends Biotechnol. 2016 Sep;34(9):733-745. (PMID: 27032730)
Front Bioeng Biotechnol. 2020 Jan 17;7:451. (PMID: 32010677)
Biomacromolecules. 2018 Sep 10;19(9):3747-3753. (PMID: 30095899)
Polymers (Basel). 2020 Apr 14;12(4):. (PMID: 32295115)
Clin Cancer Res. 2008 Sep 1;14(17):5459-65. (PMID: 18765537)
فهرسة مساهمة: Keywords: CAM assay; PCL (polycaprolactone); angiogenesis; gelatin; polyHIPE; vascularisation
تواريخ الأحداث: Date Created: 20240123 Latest Revision: 20240124
رمز التحديث: 20240124
مُعرف محوري في PubMed: PMC10800367
DOI: 10.3389/fbioe.2023.1321197
PMID: 38260750
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-4185
DOI:10.3389/fbioe.2023.1321197