دورية أكاديمية
Quantification of smooth muscle in human airways by polarization-sensitive optical coherence tomography requires correction for perichondrium.
| العنوان: | Quantification of smooth muscle in human airways by polarization-sensitive optical coherence tomography requires correction for perichondrium. |
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| المؤلفون: | Hackmann MJ; School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia.; Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia., Cairncross A; School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia.; Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Western Australia, Australia., Elliot JG; School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia.; Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Western Australia, Australia., Mulrennan S; Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia.; Institute of Respiratory Health, The University of Western Australia, Crawley, Western Australia, Australia.; Medical School, The University of Western Australia, Crawley, Western Australia, Australia., Nilsen K; Central Clinical School, Monash University, Melbourne, Victoria, Australia., Thompson BR; Melbourne School of Health Sciences, The University of Melbourne, Melbourne, Victoria, Australia., Li Q; Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia., Karnowski K; Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.; International Centre for Translational Eye Research, Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland., Sampson DD; School of Computer Science and Electronic Engineering, University of Surrey, Guildford, United Kingdom., McLaughlin RA; Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.; Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia, Australia.; Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia., Cense B; Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia.; Department of Mechanical Engineering, Yonsei University, Seoul, South Korea., James AL; Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Western Australia, Australia.; Medical School, The University of Western Australia, Crawley, Western Australia, Australia., Noble PB; School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia. |
| المصدر: | American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2024 Mar 01; Vol. 326 (3), pp. L393-L408. Date of Electronic Publication: 2024 Jan 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901229 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1504 (Electronic) Linking ISSN: 10400605 NLM ISO Abbreviation: Am J Physiol Lung Cell Mol Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Physiological Society, c1989- |
| مواضيع طبية MeSH: | Tomography, Optical Coherence*/methods , Asthma*, Humans ; Swine ; Animals ; Respiratory System ; Cartilage ; Muscle, Smooth/diagnostic imaging |
| مستخلص: | Quantifying airway smooth muscle (ASM) in patients with asthma raises the possibility of improved and personalized disease management. Endobronchial polarization-sensitive optical coherence tomography (PS-OCT) is a promising quantitative imaging approach that is in the early stages of clinical translation. To date, only animal tissues have been used to assess the accuracy of PS-OCT to quantify absolute (rather than relative) ASM in cross sections with directly matched histological cross sections as validation. We report the use of whole fresh human and pig airways to perform a detailed side-by-side qualitative and quantitative validation of PS-OCT against gold-standard histology. We matched and quantified 120 sections from five human and seven pig (small and large) airways and linked PS-OCT signatures of ASM to the tissue structural appearance in histology. Notably, we found that human cartilage perichondrium can share with ASM the properties of birefringence and circumferential alignment of fibers, making it a significant confounder for ASM detection. Measurements not corrected for perichondrium overestimated ASM content several-fold ( P < 0.001, paired t test). After careful exclusion of perichondrium, we found a strong positive correlation ( r = 0.96, P < 0.00001) of ASM area measured by PS-OCT and histology, supporting the method's application in human subjects. Matching human histology further indicated that PS-OCT allows conclusions on the intralayer composition and in turn potential contractile capacity of ASM bands. Together these results form a reliable basis for future clinical studies. NEW & NOTEWORTHY Polarization-sensitive optical coherence tomography (PS-OCT) may facilitate in vivo measurement of airway smooth muscle (ASM). We present a quantitative validation correlating absolute ASM area from PS-OCT to directly matched histological cross sections using human tissue. A major confounder for ASM quantification was observed and resolved: fibrous perichondrium surrounding hyaline cartilage in human airways presents a PS-OCT signature similar to ASM for birefringence and optic axis orientation. Findings impact the development of automated methods for ASM segmentation. |
| معلومات مُعتمدة: | APP1180854 DHAC | National Health and Medical Research Council (NHMRC); APP1141552) DHAC | National Health and Medical Research Council (NHMRC); BIOBANK2021 Future Health Research and Innovation Fund, Western Australia; MRGP22-23_18 Sir Charles Gairdner and Osborne Park Health Care Group Research Advisory Committee Project Grant; SGP21-22_30 Sir Charles Gairdner and Osborne Park Health Care Group Research Advisory Committee Project Grant; 2022H1D3A2A0109644511 Brain Pool Program, National Research Foundation of Korea; PPN/PPO/2018/1/00082/U/00001/01 Polish Returns Program, Polish National Agency for Academic Exchange; Australian Government International Research Training Program (RTP) Fee Offset Scholarships; The University of Western Australia University Postgraduate Award and Ad Hoc Top Up Scholarship; The University of Western Australia, Scholarship for International Research Fees |
| فهرسة مساهمة: | Keywords: airway smooth muscle; asthma; histology; polarization-sensitive optical coherence tomography; validation |
| تواريخ الأحداث: | Date Created: 20240123 Date Completed: 20240307 Latest Revision: 20240307 |
| رمز التحديث: | 20240307 |
| DOI: | 10.1152/ajplung.00254.2023 |
| PMID: | 38261720 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1504 |
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| DOI: | 10.1152/ajplung.00254.2023 |