دورية أكاديمية
Changes in the conflicting nongenomic effects of progesterone in rat myometrium during pregnancy.
| العنوان: | Changes in the conflicting nongenomic effects of progesterone in rat myometrium during pregnancy. |
|---|---|
| المؤلفون: | Yoshida A; Department of Obstetrics and Gynecology, Kansai Medical University, Hirakata, Osaka, Japan., Yasuda K; Department of Obstetrics and Gynecology, Kansai Medical University, Hirakata, Osaka, Japan. Electronic address: ksafety@yasuda-mf.or.jp., Okada H; Department of Obstetrics and Gynecology, Kansai Medical University, Hirakata, Osaka, Japan. |
| المصدر: | Life sciences [Life Sci] 2024 Mar 01; Vol. 340, pp. 122454. Date of Electronic Publication: 2024 Jan 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Progesterone*/pharmacology , Progesterone*/metabolism , Myometrium*/metabolism, Pregnancy ; Female ; Rats ; Animals ; Cycloheximide/pharmacology ; Cycloheximide/metabolism ; Dactinomycin/pharmacology ; Dactinomycin/metabolism ; Receptors, Progesterone/metabolism ; Progestins/pharmacology ; Uterine Contraction |
| مستخلص: | Aims: Although the functions of progesterone in the myometrium are well-established, the nongenomic effects of progesterone in pregnant myometrial contractions are still unclear. Therefore, this study aimed to investigate changes in the nongenomic effects of progesterone during pregnancy. Main Methods: Myometrial strips were obtained from non-pregnant, pregnant, and postpartum rats, and the nongenomic effects of progesterone in the myometrium during pregnancy were examined. Additionally, the influence of actinomycin D and cycloheximide and the effects of Org OD-02-0 (a specific membrane progesterone receptor (mPR) agonist) in the myometrium were investigated. Moreover, DNA microarray and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to identify genes involved in progesterone-induced effects in the myometrium. Key Findings: Progesterone did not cause rhythmic contractions in non-pregnant myometrium but induced rhythmic contractions in pregnant myometrium, with the effects peaking at 20 d + 8 h of pregnancy. However, myometrial contractions decreased after delivery and were restored to non-pregnant levels at 7 d postpartum. Additionally, progesterone stably inhibited high KCl-induced myometrial contractions during pregnancy. Moreover, the nongenomic effects of progesterone were unaffected by actinomycin D or cycloheximide, and Org OD-02-0 effectively mimicked these effects. DNA microarray analysis and qRT-PCR revealed a significant increase in mPRβ gene expression during pregnancy. However, mPRα, mPRγ, mPRδ, and mPRε expression levels remained unchanged. Significance: The stimulatory nongenomic effect of progesterone, which was inducible and mPRβ-dependent during pregnancy, may be involved in parturition. The inhibitory effect, which was constitutive and depended on other mPRs, may be involved in pregnancy maintenance. Competing Interests: Declaration of competing interest This manuscript has not been published or presented elsewhere in part or in entirety and is not under consideration by another journal. The study design was approved by the appropriate ethics review board. We have read and understood your journal's policies, and we believe that neither the manuscript nor the study violates any of these. There are no conflicts of interest to declare. (Copyright © 2024. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Membrane progesterone receptors; Myometrium; Nongenomic effects; Pregnancy; Progesterone; mPRβ |
| المشرفين على المادة: | 4G7DS2Q64Y (Progesterone) 98600C0908 (Cycloheximide) 1CC1JFE158 (Dactinomycin) 0 (Receptors, Progesterone) 0 (Progestins) |
| تواريخ الأحداث: | Date Created: 20240123 Date Completed: 20240214 Latest Revision: 20240214 |
| رمز التحديث: | 20240214 |
| DOI: | 10.1016/j.lfs.2024.122454 |
| PMID: | 38262574 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
|---|---|
| DOI: | 10.1016/j.lfs.2024.122454 |