دورية أكاديمية
أعرض في EDS

Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed.

التفاصيل البيبلوغرافية
العنوان: Integrative genomic analyses reveal putative cell type-specific targets of the Drosophila ets transcription factor Pointed.
المؤلفون: Bollepogu Raja KK; Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA., Yeung K; Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA., Shim YK; Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA., Mardon G; Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. gmardon@bcm.edu.; Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA. gmardon@bcm.edu.
المصدر: BMC genomics [BMC Genomics] 2024 Jan 23; Vol. 25 (1), pp. 103. Date of Electronic Publication: 2024 Jan 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Drosophila* , Genomics*, Animals ; Cell Differentiation ; ErbB Receptors ; Larva ; Proto-Oncogene Proteins c-ets
مستخلص: The Ets domain transcription factors direct diverse biological processes throughout all metazoans and are implicated in development as well as in tumor initiation, progression and metastasis. The Drosophila Ets transcription factor Pointed (Pnt) is the downstream effector of the Epidermal growth factor receptor (Egfr) pathway and is required for cell cycle progression, specification, and differentiation of most cell types in the larval eye disc. Despite its critical role in development, very few targets of Pnt have been reported previously. Here, we employed an integrated approach by combining genome-wide single cell and bulk data to identify putative cell type-specific Pnt targets. First, we used chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq) to determine the genome-wide occupancy of Pnt in late larval eye discs. We identified enriched regions that mapped to an average of 6,941 genes, the vast majority of which are novel putative Pnt targets. Next, we integrated ChIP-seq data with two other larval eye single cell genomics datasets (scRNA-seq and snATAC-seq) to reveal 157 putative cell type-specific Pnt targets that may help mediate unique cell type responses upon Egfr-induced differentiation. Finally, our integrated data also predicts cell type-specific functional enhancers that were not reported previously. Together, our study provides a greatly expanded list of putative cell type-specific Pnt targets in the eye and is a resource for future studies that will allow mechanistic insights into complex developmental processes regulated by Egfr signaling.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Differentiation; Drosophila eye disc; Epidermal growth factor receptor; Integrative genomics; Pointed-ChIP-seq; Single cell genomics
المشرفين على المادة: EC 2.7.10.1 (ErbB Receptors)
0 (Proto-Oncogene Proteins c-ets)
0 (pnt protein, Drosophila)
تواريخ الأحداث: Date Created: 20240123 Date Completed: 20240205 Latest Revision: 20240205
رمز التحديث: 20240205
مُعرف محوري في PubMed: PMC10807358
DOI: 10.1186/s12864-024-10017-7
PMID: 38262913
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2164
DOI:10.1186/s12864-024-10017-7