Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis.

التفاصيل البيبلوغرافية
العنوان:	Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis.
المؤلفون:	Yi SY; Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. yisy@kongju.ac.kr.; Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University, Yesan, 32439, Republic of Korea. yisy@kongju.ac.kr., Nekrasov V; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Plant Sciences and the Bioeconomy, Rothamsted Research, Harpenden, UK., Ichimura K; Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan., Kang SY; Department of Horticulture, College of Industrial Sciences, Kongju National University, Yesan, 32439, Republic of Korea. sykang@kongju.ac.kr.; Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University, Yesan, 32439, Republic of Korea. sykang@kongju.ac.kr., Shirasu K; Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan. ken.shirasu@riken.jp.
المصدر:	Plant molecular biology [Plant Mol Biol] 2024 Jan 24; Vol. 114 (1), pp. 7. Date of Electronic Publication: 2024 Jan 24.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Kluwer Academic Country of Publication: Netherlands NLM ID: 9106343 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-5028 (Electronic) Linking ISSN: 01674412 NLM ISO Abbreviation: Plant Mol Biol Subsets: MEDLINE
أسماء مطبوعة:	Publication: Dordrecht : Kluwer Academic Original Publication: The Hague ; Boston : Martinus Nijhoff/Dr. W. Junk, 1981-
مواضيع طبية MeSH:	Innate Immunity Recognition* , Arabidopsis*, Immunity, Innate ; Plant Proteins ; Penicillin V ; Ligases
مستخلص:	Key Message: Plant U-box E3 ligases PUB20 and PUB21 are flg22-triggered signaling components and negatively regulate immune responses. Plant U-box proteins (PUBs) constitute a class of E3 ligases that are associated with various stress responses. Among the class IV PUBs featuring C-terminal Armadillo (ARM) repeats, PUB20 and PUB21 are closely related homologs. Here, we show that both PUB20 and PUB21 negatively regulate innate immunity in plants. Loss of PUB20 and PUB21 function leads to enhanced resistance to surface inoculation with the virulent bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). However, the resistance levels remain unaffected after infiltration inoculation, suggesting that PUB20 and PUB21 primarily function during the early defense stages. The enhanced resistance to Pst DC3000 in PUB mutant plants (pub20-1, pub21-1, and pub20-1/pub21-1) correlates with extensive flg22-triggered reactive oxygen production, strong MPK3 activation, and enhanced transcriptional activation of early immune response genes. Additionally, PUB mutant plants (except pub21-1) exhibit constitutive stomatal closure after Pst DC3000 inoculation, implying the significant role of PUB20 in stomatal immunity. Comparative analyses of flg22 responses between PUB mutants and wild-type plants reveals that the robust activation of the pattern-induced immune responses may enhance resistance against Pst DC3000. Notably, the hypersensitivity responses triggered by RPM1/avrRpm1 and RPS2/avrRpt2 are independent of PUB20 and PUB21. These results suggest that PUB20 and PUB21 knockout mutations affect bacterial invasion, likely during the early stages, acting as negative regulators of plant immunity. (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
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معلومات مُعتمدة:	NRF-2018K1A3A7A03089858 Ministry of Education of the Republic of Korea and National Research Foundation of Korea; 22H00364 KAKENHI
فهرسة مساهمة:	Keywords: Arabidopsis; E3 ligase; PUB20; PUB21; Pattern-triggered immunity
المشرفين على المادة:	0 (Plant Proteins) Z61I075U2W (Penicillin V) EC 6.- (Ligases)
تواريخ الأحداث:	Date Created: 20240124 Date Completed: 20240125 Latest Revision: 20240219
رمز التحديث:	20240219
DOI:	10.1007/s11103-023-01409-6
PMID:	38265485
قاعدة البيانات:	MEDLINE

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تدمد:	1573-5028
DOI:	10.1007/s11103-023-01409-6