دورية أكاديمية
أعرض في EDS

Characterizing adjuvants' effects at murine immunoglobulin repertoire level.

التفاصيل البيبلوغرافية
العنوان: Characterizing adjuvants' effects at murine immunoglobulin repertoire level.
المؤلفون: Feng F; Department of Microbiology, Boston University, Boston, MA 02118, USA., Yuen R; Department of Microbiology, Boston University, Boston, MA 02118, USA., Wang Y; Department of Microbiology, Boston University, Boston, MA 02118, USA., Hua A; Department of Microbiology, Boston University, Boston, MA 02118, USA., Kepler TB; Department of Microbiology, Boston University, Boston, MA 02118, USA.; Department of Mathematics and Statistics, Boston University, Boston, MA 02118, USA., Wetzler LM; Department of Microbiology, Boston University, Boston, MA 02118, USA.; Department of Medicine, Boston University School of Medicine, Boston University, Boston, MA 02118, USA.
المصدر: IScience [iScience] 2023 Dec 14; Vol. 27 (1), pp. 108749. Date of Electronic Publication: 2023 Dec 14 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2018]-
مستخلص: Generating large-scale, high-fidelity sequencing data is challenging and, furthermore, not much has been done to characterize adjuvants' effects at the repertoire level. Thus, we introduced an IgSeq pipeline that standardized library prep protocols and data analysis functions for accurate repertoire profiling. We then studied systemically effects of CpG and Alum on the Ig heavy chain repertoire using the ovalbumin (OVA) murine model. Ig repertoires of different tissues (spleen and bone marrow) and isotypes (IgG and IgM) were examined and compared in IGHV mutation, gene usage, CDR3 length, clonal diversity, and clonal selection. We found Ig repertoires of different compartments exhibited distinguishable profiles at the non-immunized steady state, and distinctions became more pronounced upon adjuvanted immunizations. Notably, Alum and CpG effects exhibited different tissue- and isotype-preferences. The former led to increased diversity of abundant clones in bone marrow, and the latter promoted the selection of IgG clones in both tissues.
Competing Interests: The authors declare that there is no conflict of interest.
(© 2023.)
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معلومات مُعتمدة: R01 AI040944 United States AI NIAID NIH HHS; U19 AI117892 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: Genomic analysis; Immunology; Machine learning; Sequence analysis
تواريخ الأحداث: Date Created: 20240125 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10805652
DOI: 10.1016/j.isci.2023.108749
PMID: 38269092
قاعدة البيانات: MEDLINE
الوصف
تدمد:2589-0042
DOI:10.1016/j.isci.2023.108749