دورية أكاديمية
أعرض في EDS

Targeting NF-κB/COX-2 signaling by soyasaponin I alleviates diclofenac-induced gastric ulceration in male albino rats.

التفاصيل البيبلوغرافية
العنوان: Targeting NF-κB/COX-2 signaling by soyasaponin I alleviates diclofenac-induced gastric ulceration in male albino rats.
المؤلفون: Morsi AA; Department of Histology and Cell Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Shawky LM; Department of Histology and Cell Biology, Faculty of Medicine, Benha University, Benha, Egypt., Shawky TM; Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Giza, Egypt.; Department of Basic Medical Sciences, Vision Colleges, Riyadh, Saudi Arabia., Bahr MH; Department of Basic Medical Sciences, Vision Colleges, Riyadh, Saudi Arabia.; Department of Pharmacology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Alnasr MTA; College of Medicine, Alfaisal University, Riyadh, Saudia Arabia., El Bana E; Department of Anatomy, Faculty of Medicine, Benha University, Benha, Egypt.
المصدر: Cell biochemistry and function [Cell Biochem Funct] 2024 Jan; Vol. 42 (1), pp. e3927.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8305874 Publication Model: Print Cited Medium: Internet ISSN: 1099-0844 (Electronic) Linking ISSN: 02636484 NLM ISO Abbreviation: Cell Biochem Funct Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford, England : Wiley-Blackwell
Original Publication: Guildford, Surrey : Butterworth Scientific Ltd., c1983-
مواضيع طبية MeSH: NF-kappa B* , Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Phenylenediamines* , Saponins*, Oleanolic Acid/*analogs & derivatives, Male ; Animals ; Rats ; Rats, Wistar ; Cyclooxygenase 2 ; Diclofenac ; Ulcer ; Ranitidine ; Dinoprostone ; Ki-67 Antigen ; Eosine Yellowish-(YS)
مستخلص: Gastric ulceration is a prevalent worldwide clinical presentation due to altered gastric defense mechanisms. Nonsteroidal anti-inflammatory drugs are one of the common causes of gastric ulcers mediated by the release of inflammatory mediators. The study aimed to investigate the potential protective effect of soyasaponin I (soya) against diclofenac (DIC)-induced gastric ulcer in rats and to highlight the underlying mechanisms. The experiment was conducted on 40 male Wistar albino rats, equally distributed into five groups: control, DIC-induced ulcer (9 mg/kg/d, orally, twice daily for 3 days), ulcer/soya-, ulcer/ranitidine-, and ulcer/soya/selective nuclear factor kappa B inhibitor (JSH-23)-treated groups. The doses of soya, ranitidine, and JSH were 20, 25, and 5 mg/kg/d, respectively, given orally. Gastric specimens were prepared for gene and histological study and for biochemical analysis of gastric prostaglandin E2 (PGE2), oxidative markers, and inflammatory cytokines. The gastric samples were formalin-fixed, paraffin-embedded, and subjected to hematoxylin and eosin (H&E), PAS staining, and immunohistochemical assay for identification of nuclear factor kappa B (NF-κB), cyclooxygenase-2 (COX-2), and proliferation marker (Ki67) expressions. The findings revealed decreased gastric PGE2 and altered inflammatory and oxidative markers in the ulcer model group. The H&E staining showed mucosal injury characterized by mucosal surface defects and inflammatory cell infiltrations. The polymerase chain reaction (PCR) and immunohistochemistry demonstrated an upregulation of NF-κB and COX-2 expression at gene/protein levels; meanwhile, Ki67 downregulation. The soya-treated group showed maintained biochemical, histological, and PCR findings comparable to the ranitidine-treated group. The JSH-23-treated group still showed partial gastric protection with biochemical and immunohistochemical changes. Soyasaponin I ameliorated DIC-induced gastric ulcers by targeting the COX-2 activity through modulation of NF-κB signaling.
(© 2024 John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: immunohistochemistry; inflammation; oxidative stress; phytochemicals; rat stomach
المشرفين على المادة: 0 (NF-kappa B)
0 (4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine)
EC 1.14.99.1 (Cyclooxygenase 2)
51330-27-9 (soyasaponin I)
144O8QL0L1 (Diclofenac)
884KT10YB7 (Ranitidine)
K7Q1JQR04M (Dinoprostone)
0 (Ki-67 Antigen)
TDQ283MPCW (Eosine Yellowish-(YS))
6SMK8R7TGJ (Oleanolic Acid)
0 (Phenylenediamines)
0 (Saponins)
تواريخ الأحداث: Date Created: 20240125 Date Completed: 20240126 Latest Revision: 20240126
رمز التحديث: 20240126
DOI: 10.1002/cbf.3927
PMID: 38269501
قاعدة البيانات: MEDLINE
الوصف
تدمد:1099-0844
DOI:10.1002/cbf.3927