دورية أكاديمية
أعرض في EDS

Assessing viral freshwater hazard using a toxicokinetic model and Dreissena polymorpha.

التفاصيل البيبلوغرافية
العنوان: Assessing viral freshwater hazard using a toxicokinetic model and Dreissena polymorpha.
المؤلفون: Do Nascimento J; UMR-I 02 SEBIO (Stress Environnementaux et BIOsurveillance des milieux aquatiques), Université de Reims Champagne Ardenne, UFR Sciences Exactes et Naturelles, Campus Moulin de Housse, BP 1039, 51687, Reims cedex 2, France., Palos Ladeiro M; UMR-I 02 SEBIO (Stress Environnementaux et BIOsurveillance des milieux aquatiques), Université de Reims Champagne Ardenne, UFR Sciences Exactes et Naturelles, Campus Moulin de Housse, BP 1039, 51687, Reims cedex 2, France., Bonnard I; UMR-I 02 SEBIO (Stress Environnementaux et BIOsurveillance des milieux aquatiques), Université de Reims Champagne Ardenne, UFR Sciences Exactes et Naturelles, Campus Moulin de Housse, BP 1039, 51687, Reims cedex 2, France., Gantzer C; LCPME UMR 7564, Université de Lorraine - CNRS, 405 rue de Vandoeuvre, 54600, Villers-lès-Nancy, France., Boudaud N; Food Safety Department, Actalia, Saint-Lô, F-50000, France., Lopes C; Université de Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, Villeurbanne, 69622, France., Geffard A; UMR-I 02 SEBIO (Stress Environnementaux et BIOsurveillance des milieux aquatiques), Université de Reims Champagne Ardenne, UFR Sciences Exactes et Naturelles, Campus Moulin de Housse, BP 1039, 51687, Reims cedex 2, France. Electronic address: alain.geffard@univ-reims.fr.
المصدر: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Mar 01; Vol. 344, pp. 123420. Date of Electronic Publication: 2024 Jan 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8804476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6424 (Electronic) Linking ISSN: 02697491 NLM ISO Abbreviation: Environ Pollut Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Barking, Essex, England : Elsevier Applied Science Publishers, c1987-
مواضيع طبية MeSH: Dreissena* , Bivalvia*, Animals ; Toxicokinetics ; Fresh Water/chemistry ; Water
مستخلص: The detection all pathogenic enteric viruses in water is expensive, time-consuming, and limited by numerous technical difficulties. Consequently, using reliable indicators such as F-specific RNA phages (FRNAPH) can be well adapted to assess the risk of viral contamination of fecal origin in surface waters. However, the variability of results inherent to the water matrix makes it difficult to use them routinely and to interpret viral risk. Spatial and temporal variability of surface waters can lead to underestimate this risk, in particular in the case of low loading. The use of bivalve mollusks as accumulating systems appears as a promising alternative, as recently highlighted with the freshwater mussel Dreissena polymorpha, but its capacity to accumulate and depurate FRNAPH needs to be better understood and described. The purpose of this study is to characterise the kinetics of accumulation and elimination of infectious FRNAPH by D. polymorpha in laboratory conditions, formalised by a toxico-kinetic (TK) mechanistic model. Accumulation and depuration experiments were performed at a laboratory scale to determine the relationship between the concentration of infectious FRNAPH in water and the concentration accumulated by D. polymorpha. The mussels accumulated infectious FRNAPH (3-5.4 × 10 4  PFU/g) in a fast and concentration-dependent way in only 48 h, as already recently demonstrated. The second exposure demonstrated that the kinetics of infectious FRNAPH depuration by D. polymorpha was independent to the exposure dose, with a T 90 (time required to depurate 90 % of the accumulated concentration) of approximately 6 days. These results highlight the capacities of D. polymorpha to detect and reflect the viral pollution in an integrative way and over time, which is not possible with point water sampling. Different TK models were fitted based on the concentrations measured in the digestive tissues (DT) of D. polymorpha. The model has been developed to formalise the kinetics of phage accumulation in mussels tissues through the simultaneous estimation of accumulation and depuration rates. This model showed that accumulation depended on the exposure concentration, while depuration did not. Standardized D. polymorpha could be easily transplanted to the environment to predict viral concentrations using the TK model defined in the present study to predict the level of contamination of bodies of water on the basis of the level of phages accumulated by the organisms. It will be also provide a better understanding of the dynamics of the virus in continental waters at different time and spatial scales, and thereby contribute to the protection of freshwater resources.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Accumulation; Depuration; F-specific RNA bacteriophages; Modelling; Zebra mussel
المشرفين على المادة: 059QF0KO0R (Water)
تواريخ الأحداث: Date Created: 20240125 Date Completed: 20240226 Latest Revision: 20240226
رمز التحديث: 20240226
DOI: 10.1016/j.envpol.2024.123420
PMID: 38272165
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-6424
DOI:10.1016/j.envpol.2024.123420