دورية أكاديمية
Effect of Cyclosporine A on Th1/Th2 Cytokine Production by Decidual Stromal Cells Mediated by Trophoblast-derived Galectin-9.
العنوان: | Effect of Cyclosporine A on Th1/Th2 Cytokine Production by Decidual Stromal Cells Mediated by Trophoblast-derived Galectin-9. |
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المؤلفون: | Mei J; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Wu B; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Li M; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Ma L; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Yang X; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Ma Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China. mayanlinma@hotmail.com.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China. mayanlinma@hotmail.com.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com., Huang Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China. huangyh4180@hainmc.edu.cn.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China. huangyh4180@hainmc.edu.cn.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn. |
المصدر: | Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Jul; Vol. 31 (7), pp. 1903-1914. Date of Electronic Publication: 2024 Jan 25. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2020- : [New York] : Springer Original Publication: Thousand Oaks, Calif. : Sage |
مواضيع طبية MeSH: | Galectins*/metabolism , Trophoblasts*/metabolism , Trophoblasts*/drug effects , Cyclosporine*/pharmacology , Cytokines*/metabolism , Th1 Cells*/drug effects , Th1 Cells*/metabolism , Th1 Cells*/immunology , Decidua*/metabolism , Decidua*/drug effects , Decidua*/cytology , Th2 Cells*/drug effects , Th2 Cells*/metabolism , Th2 Cells*/immunology , Stromal Cells*/drug effects , Stromal Cells*/metabolism, Humans ; Female ; Pregnancy ; Hepatitis A Virus Cellular Receptor 2/metabolism ; Cell Line ; Cells, Cultured ; Immunosuppressive Agents/pharmacology ; Signal Transduction/drug effects |
مستخلص: | This study aimed to investigate the effect of cyclosporine A (CsA) on secretion of Th1 and Th2 cytokines by decidual stromal cells (DSCs) mediated by galectin (Gal)-9.HTR8/SVneo cells and primary trophoblasts were used for in vitro studies. Gal-9 expression was measured using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, CsA was used to regulate Gal-9 expression in trophoblasts. DSCs were treated with trophoblast supernatant and changes in Th1 and Th2 cytokine levels were analyzed. Changes in DSC levels of the T-cell immunoglobulin mucin receptor 3 (TIM-3) levels in DSCs after treatment with Gal-9 were assessed. Western blotting and ERK and AKT inhibitors were used to assess the involvement of the corresponding signaling pathways. Gal-9 was expressed by both primary trophoblasts and HTR8/SVneo cells. CsA treatment increased Gal-9 secretion by trophoblasts, which in turn increased IL-6 (Th2 cytokine) and decreased TNF-α and IFN-γ (Th1 cytokines) secretion in DSCs. Upon downregulation of trophoblast Gal-9 secretion, DSCs secreted lower levels of Th2 cytokines and higher levels of Th1 cytokines, and the effect was reversed by addition of CsA. TIM-3 expression changed in parallel with Gal-9 secretion. CsA treatment upregulated expression of Gal-9 in trophoblasts, promoted secretion of Th2 cytokines, and inhibited secretion of Th1 cytokines via ERK signaling. (© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.) |
References: | Xu YY, Wang SC, Li DJ, et al. Co-signaling molecules in maternal-fetal immunity. Trends Mol Med. 2017;23(1):46–58. (PMID: 27914866) Szekeres-Bartho J. Immunological relationship between the mother and the fetus. Int Rev Immunol. 2002;21(6):471–95. (PMID: 12650238) Specht S, Arriens S, Hoerauf A. Induction of chronic colitis in IL-10 deficient mice requires IL-4. Microbes Infect. 2006;8(3):694–703. (PMID: 16513385) Dresser DW. The potentiating effect of pregnancy on humoral immune responses of mice. J Reprod Immunol. 1991;20(3):253–66. (PMID: 1960706) Shiu MH, Schottenfeld D, Maclean B, et al. Adverse effect of pregnancy on melanoma: a reappraisal. Cancer. 1976;37(1):181–7. (PMID: 1247953) Cloke B, Huhtinen K, Fusi L, et al. The androgen and progesterone receptors regulate distinct gene networks and cellular functions in decidualizing endometrium. Endocrinology. 2008;149(9):4462–74. (PMID: 185115035393297) Mor G, Cardenas I. The immune system in pregnancy: a unique complexity. Am J Reprod Immunol. 2010;63(6):425–33. (PMID: 203676293025805) Khosravi M, Ryan W, Webster DA, et al. Variation of oxygen requirement with plasmid size in recombinant Escherichia coli. Plasmid. 1990;23(2):138–43. (PMID: 2194228) Zhou WH, Du MR, Dong L, et al. Chemokine CXCL12 promotes the cross-talk between trophoblasts and decidual stromal cells in human first-trimester pregnancy. Hum Reprod. 2008;23(12):2669–79. (PMID: 18687671) Oreshkova T, Dimitrov R, Mourdjeva M. A cross-talk of decidual stromal cells, trophoblast, and immune cells: a prerequisite for the success of pregnancy. Am J Reprod Immunol. 2012;68(5):366–73. (PMID: 22672047) Zhu C, Anderson AC, Schubart A, et al. The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity. Nat Immunol. 2005;6(12):1245–52. (PMID: 16286920) Sanchez-Fueyo A, Tian J, Picarella D, et al. Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance. Nat Immunol. 2003;4(11):1093–101. (PMID: 14556005) Lu X, McCoy KS, Xu J, et al. Galectin-9 ameliorates respiratory syncytial virus-induced pulmonary immunopathology through regulating the balance between Th17 and regulatory T cells. Virus Res. 2015;195:162–71. (PMID: 25451068) Li YH, Zhou WH, Tao Y, et al. The galectin-9/Tim-3 pathway is involved in the regulation of NK cell function at the maternal-fetal interface in early pregnancy. Cell Mol Immunol. 2016;13(1):73–81. (PMID: 25578313) Li ZH, Wang LL, Liu H, et al. Galectin-9 Alleviates LPS-induced preeclampsia-like impairment in rats via switching decidual macrophage polarization to M2 subtype. Front Immunol. 2018;9:3142. (PMID: 30687334) Nulman I, Sgro M, Barrera M, et al. Long-term neurodevelopment of children exposed in utero to ciclosporin after maternal renal transplant. Paediatr Drugs. 2010;12(2):113–22. (PMID: 20095652) Little MA, Abraham KA, Kavanagh J, et al. Pregnancy in Irish renal transplant recipients in the cyclosporine era. Ir J Med Sci. 2000;169(1):19–21. (PMID: 10846851) Ling Y, Huang Y, Chen C, et al. Low dose Cyclosporin A treatment increases live birth rate of unexplained recurrent abortion - initial cohort study. Clin Exp Obstet Gynecol. 2017;44(2):230–5. (PMID: 29746028) Huang YH, Ma YL, Ma L, et al. Cyclosporine A improves adhesion and invasion of mouse preimplantation embryos via upregulating integrin beta3 and matrix metalloproteinase-9. Int J Clin Exp Pathol. 2014;7(4):1379–88. (PMID: 248179344014218) Huang W, Lu W, Li Q, et al. Effects of cyclosporine A on proliferation, invasion and migration of HTR-8/SVneo human extravillous trophoblasts. Biochem Biophys Res Commun. 2020;533(4):645–50. (PMID: 33032826) Tang CL, Zhao HB, Li MQ, et al. Focal adhesion kinase signaling is necessary for the Cyclosporin A-enhanced migration and invasion of human trophoblast cells. Placenta. 2012;33(9):704–11. (PMID: 22766276) Du MR, Dong L, Zhou WH, et al. Cyclosporin a improves pregnancy outcome by promoting functions of trophoblasts and inducing maternal tolerance to the allogeneic fetus in abortion-prone matings in the mouse. Biol Reprod. 2007;76(5):906–14. (PMID: 17229932) Wei Y, Zhou X, Huang W, et al. Generation of trophoblast-like cells from the amnion in vitro: a novel cellular model for trophoblast development. Placenta. 2017;51:28–37. (PMID: 28292466) James JL, Stone PR, Chamley LW. The isolation and characterization of a population of extravillous trophoblast progenitors from first trimester human placenta. Hum Reprod. 2007;22(8):2111–9. (PMID: 17580299) Jie Q, Sun F, Li Q, et al. Downregulated ribosomal protein L39 inhibits trophoblast cell migration and invasion by targeting E-cadherin in the placenta of patients with preeclampsia. FASEB J. 2021;35(4):e21322. (PMID: 33710681) El HH, Crepaux V, May-Panloup P, et al. Recurrent pregnancy loss: current perspectives. Int J Womens Health. 2017;9:331–45. Fraccaroli L, Alfieri J, Larocca L, et al. A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation. Hum Reprod. 2009;24(1):166–75. (PMID: 18824472) Wallace AE, Fraser R, Cartwright JE. Extravillous trophoblast and decidual natural killer cells: a remodelling partnership. Hum Reprod Update. 2012;18(4):458–71. (PMID: 225231093373213) Ban Y, Zhao Y, Liu F, et al. Effect of indoleamine 2,3-dioxygenase expressed in HTR-8/SVneo Cells on decidual NK cell cytotoxicity. Am J Reprod Immunol. 2016;75(5):519–28. (PMID: 26782048) Chakir H, Wang H, Lefebvre DE, et al. T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3. J Immunol Methods. 2003;278(1–2):157–69. (PMID: 12957404) Yang H, Qiu L, Chen G, et al. Proportional change of CD4+CD25+ regulatory T cells in decidua and peripheral blood in unexplained recurrent spontaneous abortion patients. Fertil Steril. 2008;89(3):656–61. (PMID: 17543960) Piao HL, Wang SC, Tao Y, et al. Cyclosporine A enhances Th2 bias at the maternal-fetal interface in early human pregnancy with aid of the interaction between maternal and fetal cells. PLoS ONE. 2012;7(9):e45275. (PMID: 230289013459906) Calleja-Agius J, Muttukrishna S, Pizzey AR, et al. Pro- and antiinflammatory cytokines in threatened miscarriages. Am J Obstet Gynecol. 2011;205(1):83–8. Dubinsky V, Junovich G, Gentile T, et al. IL-6 as a regulatory factor of the humoral response during pregnancy. Am J Reprod Immunol. 2008;60(3):197–203. (PMID: 18652576) Chen L, Flies DB. Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 2013;13(4):227–42. (PMID: 234703213786574) Han G, Chen G, Shen B, et al. Tim-3: an activation marker and activation limiter of innate immune cells. Front Immunol. 2013;4:449. (PMID: 243398283857553) Li M, Peng X, Qian J, et al. Galectin-9 regulates HTR8/SVneo function via JNK signaling. Reproduction. 2021;161(1):1–10. (PMID: 33112295) Wang S, Cao C, Piao H, et al. Tim-3 protects decidual stromal cells from toll-like receptor-mediated apoptosis and inflammatory reactions and promotes Th2 bias at the maternal-fetal interface. Sci Rep. 2015;5:9013. (PMID: 257576694355741) Zhou WH, Dong L, Du MR, et al. Cyclosporin A improves murine pregnancy outcome in abortion-prone matings: involvement of CD80/86 and CD28/CTLA-4. Reproduction. 2008;135(3):385–95. (PMID: 18299432) Hu X, Zhu Q, Wang Y, et al. Newly characterized decidual Tim-3+ Treg cells are abundant during early pregnancy and driven by IL-27 coordinately with Gal-9 from trophoblasts. Hum Reprod. 2020;35(11):2454–66. (PMID: 331075658463095) Yuan J, Dong X, Yap J, et al. The MAPK and AMPK signalings: interplay and implication in targeted cancer therapy. J Hematol Oncol. 2020;13(1):113. (PMID: 328072257433213) Li Y, Zhang J, Zhang D, et al. Tim-3 signaling in peripheral NK cells promotes maternal-fetal immune tolerance and alleviates pregnancy loss. Sci Signal. 2017;10(498). |
معلومات مُعتمدة: | ZDKJ2021037 Key Research and Development Project of Hainan Province; 822MS175 the Natural Science Fodundation of Hainan Province; 82201874 the Nation Natural Science Foundation of China |
فهرسة مساهمة: | Keywords: Cyclosporine A; Decidual stromal cell; Galectin-9; Th1 cytokine; Th2 cytokine; Trophoblast |
المشرفين على المادة: | 0 (Galectins) 83HN0GTJ6D (Cyclosporine) 0 (LGALS9 protein, human) 0 (Cytokines) 0 (Hepatitis A Virus Cellular Receptor 2) 0 (HAVCR2 protein, human) 0 (Immunosuppressive Agents) |
تواريخ الأحداث: | Date Created: 20240125 Date Completed: 20240701 Latest Revision: 20240723 |
رمز التحديث: | 20240723 |
DOI: | 10.1007/s43032-023-01431-5 |
PMID: | 38273122 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1933-7205 |
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DOI: | 10.1007/s43032-023-01431-5 |