دورية أكاديمية
Effect of Cyclosporine A on Th1/Th2 Cytokine Production by Decidual Stromal Cells Mediated by Trophoblast-derived Galectin-9.
| العنوان: | Effect of Cyclosporine A on Th1/Th2 Cytokine Production by Decidual Stromal Cells Mediated by Trophoblast-derived Galectin-9. |
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| المؤلفون: | Mei J; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Wu B; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Li M; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Ma L; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Yang X; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China., Ma Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China. mayanlinma@hotmail.com.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China. mayanlinma@hotmail.com.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. mayanlinma@hotmail.com., Huang Y; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University) , Ministry of EducationThe First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn.; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, China. huangyh4180@hainmc.edu.cn.; National Center for International Research 'China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease' By the Ministry of Science and Technology of China, Haikou, China. huangyh4180@hainmc.edu.cn.; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, China. huangyh4180@hainmc.edu.cn. |
| المصدر: | Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Jul; Vol. 31 (7), pp. 1903-1914. Date of Electronic Publication: 2024 Jan 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [New York] : Springer Original Publication: Thousand Oaks, Calif. : Sage |
| مواضيع طبية MeSH: | Galectins*/metabolism , Trophoblasts*/metabolism , Trophoblasts*/drug effects , Cyclosporine*/pharmacology , Cytokines*/metabolism , Th1 Cells*/drug effects , Th1 Cells*/metabolism , Th1 Cells*/immunology , Decidua*/metabolism , Decidua*/drug effects , Decidua*/cytology , Th2 Cells*/drug effects , Th2 Cells*/metabolism , Th2 Cells*/immunology , Stromal Cells*/drug effects , Stromal Cells*/metabolism, Humans ; Female ; Pregnancy ; Hepatitis A Virus Cellular Receptor 2/metabolism ; Cell Line ; Cells, Cultured ; Immunosuppressive Agents/pharmacology ; Signal Transduction/drug effects |
| مستخلص: | This study aimed to investigate the effect of cyclosporine A (CsA) on secretion of Th1 and Th2 cytokines by decidual stromal cells (DSCs) mediated by galectin (Gal)-9.HTR8/SVneo cells and primary trophoblasts were used for in vitro studies. Gal-9 expression was measured using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, CsA was used to regulate Gal-9 expression in trophoblasts. DSCs were treated with trophoblast supernatant and changes in Th1 and Th2 cytokine levels were analyzed. Changes in DSC levels of the T-cell immunoglobulin mucin receptor 3 (TIM-3) levels in DSCs after treatment with Gal-9 were assessed. Western blotting and ERK and AKT inhibitors were used to assess the involvement of the corresponding signaling pathways. Gal-9 was expressed by both primary trophoblasts and HTR8/SVneo cells. CsA treatment increased Gal-9 secretion by trophoblasts, which in turn increased IL-6 (Th2 cytokine) and decreased TNF-α and IFN-γ (Th1 cytokines) secretion in DSCs. Upon downregulation of trophoblast Gal-9 secretion, DSCs secreted lower levels of Th2 cytokines and higher levels of Th1 cytokines, and the effect was reversed by addition of CsA. TIM-3 expression changed in parallel with Gal-9 secretion. CsA treatment upregulated expression of Gal-9 in trophoblasts, promoted secretion of Th2 cytokines, and inhibited secretion of Th1 cytokines via ERK signaling. (© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.) |
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| معلومات مُعتمدة: | ZDKJ2021037 Key Research and Development Project of Hainan Province; 822MS175 the Natural Science Fodundation of Hainan Province; 82201874 the Nation Natural Science Foundation of China |
| فهرسة مساهمة: | Keywords: Cyclosporine A; Decidual stromal cell; Galectin-9; Th1 cytokine; Th2 cytokine; Trophoblast |
| المشرفين على المادة: | 0 (Galectins) 83HN0GTJ6D (Cyclosporine) 0 (LGALS9 protein, human) 0 (Cytokines) 0 (Hepatitis A Virus Cellular Receptor 2) 0 (HAVCR2 protein, human) 0 (Immunosuppressive Agents) |
| تواريخ الأحداث: | Date Created: 20240125 Date Completed: 20240701 Latest Revision: 20240723 |
| رمز التحديث: | 20240723 |
| DOI: | 10.1007/s43032-023-01431-5 |
| PMID: | 38273122 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. | تدمد: | 1933-7205 |
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| DOI: | 10.1007/s43032-023-01431-5 |