دورية أكاديمية
Abdominal venous thromboses: detection of the JAK2 p.V617F mutation by next-generation ultradeep sequencing-A prevalence study of patients in Mecklenburg-West Pomerania (2017-2021).
| العنوان: | Abdominal venous thromboses: detection of the JAK2 p.V617F mutation by next-generation ultradeep sequencing-A prevalence study of patients in Mecklenburg-West Pomerania (2017-2021). |
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| المؤلفون: | Henze L; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Grunwald L; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Felser S; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Witte M; Department of General, Visceral, Thoracic, Vascular, and Transplant Surgery, Rostock University Medical Center, Rostock, Germany., Grosse-Thie C; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Roolf C; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Murua Escobar H; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany., Junghanss C; Department of Medicine, Clinic III-Hematology, Oncology, and Palliative Medicine, Rostock University Medical Center, Rostock, Germany. |
| المصدر: | Frontiers in medicine [Front Med (Lausanne)] 2024 Jan 11; Vol. 10, pp. 1344769. Date of Electronic Publication: 2024 Jan 11 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101648047 Publication Model: eCollection Cited Medium: Print ISSN: 2296-858X (Print) Linking ISSN: 2296858X NLM ISO Abbreviation: Front Med (Lausanne) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne, Switzerland : Frontiers Media S.A., [2014]- |
| مستخلص: | Background: Abdominal venous thromboses are rare thrombotic events with heterogeneous etiologies. They are related to myeloproliferative neoplasms (MPNs) in some patients and can occur as first signs of the disease. MPNs are characterized by mutations in the genes of Janus kinase 2 (JAK2), myeloproliferative leukemia virus oncogene (MPL), and calreticulin (CALR). Methods: Within the prospective trial "Prevalence of JAK2 mutations in patients with abdominal venous thromboses" (JAK2 MV study; German Clinical Trials Register: DRKS00026943), the peripheral blood of patients with abdominal venous thromboses in Mecklenburg-West Pomerania, a federal state located in north-east Germany, was analyzed by next-generation ultradeep sequencing for MPN-associated mutations. Clinical characteristics and blood cell counts were also of interest. The primary endpoint was the detection of the mutation JAK2 p.V617F. Secondary endpoints were the detection of other acquired variants of JAK2, as well as MPL and CALR. Results: A total of 68 patients with abdominal venous thromboses were included from February 2017 to January 2021, with splanchnic veins affected in 65 patients. The mutation JAK2 p.V617F was present in 13 patients (19%), with four patients showing low variant allele frequencies (VAF 0.1% to 1.9%). The time interval from the thrombotic event to analysis was longer for patients with the mutation. The mutation MPL p.W515R was detected in three cases, all of them with low VAF. One patient among them had a concurrent mutation of JAK2 p.V617F. The mutations CALR type I or type II were not found. Discussion: By analyzing peripheral blood for the mutation JAK2 p.V617F, an important cause of these rare thrombotic events can be identified. The development of a diagnostic workup with next-generation ultradeep sequencing for the analysis of the JAK2 p.V617F mutation and further mutations has the potential to better understand the etiology of abdominal venous thromboses in individual patients in regional clinical care, as abdominal venous thromboses are diagnosed by various medical disciplines. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Henze, Grunwald, Felser, Witte, Grosse-Thie, Roolf, Murua Escobar and Junghanss.) |
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| فهرسة مساهمة: | Keywords: JAK2 mutation; abdominal venous thromboses; anticoagulation; splanchnic vein thrombosis; ultradeep sequencing |
| تواريخ الأحداث: | Date Created: 20240126 Latest Revision: 20240128 |
| رمز التحديث: | 20240128 |
| مُعرف محوري في PubMed: | PMC10808308 |
| DOI: | 10.3389/fmed.2023.1344769 |
| PMID: | 38274463 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-858X |
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| DOI: | 10.3389/fmed.2023.1344769 |