دورية أكاديمية
Effects of a genetically modified corn hybrid with α-amylase and storage length on fermentation profile and starch disappearance of whole-plant corn silage and earlage.
| العنوان: | Effects of a genetically modified corn hybrid with α-amylase and storage length on fermentation profile and starch disappearance of whole-plant corn silage and earlage. |
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| المؤلفون: | Heinzen C Jr; Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706., Pupo MR; Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706., Ghizzi LG; Department of Animal Sciences, University of Florida, Gainesville, FL 32611., Diepersloot EC; Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706., Ferraretto LF; Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706. Electronic address: ferraretto@wisc.edu. |
| المصدر: | Journal of dairy science [J Dairy Sci] 2024 Jun; Vol. 107 (6), pp. 3631-3641. Date of Electronic Publication: 2024 Jan 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Dairy Science Association Country of Publication: United States NLM ID: 2985126R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3198 (Electronic) Linking ISSN: 00220302 NLM ISO Abbreviation: J Dairy Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Champaign, IL : American Dairy Science Association Original Publication: Lancaster, Pa. [etc.] |
| مواضيع طبية MeSH: | Zea mays* , Fermentation* , alpha-Amylases*/metabolism , Starch*/metabolism , Silage*, Plants, Genetically Modified ; Animals |
| مستخلص: | Two experiments were conducted to evaluate the effects of a genetically modified corn hybrid with α-amylase expressed in the kernel (AMY) on fermentation profile, aerobic stability, nutrient composition, and starch disappearance of whole-plant corn silage (WPCS) and earlage. Both hybrids, AMY and an isogenic corn hybrid (ISO), were grown in 10 replicated plots (5 for WPCS and 5 for earlage). Samples of each plot were collected at harvest, homogenized, and divided into 5 subsamples which were randomly assigned to 5 storage lengths (0, 30, 60, 90, and 120 d). Both datasets (WPCS and earlage), were analyzed separately as a completely randomized block design in a factorial arrangement of treatments, with a model including the fixed effects of hybrid, storage length, and their interaction, and the random effect of block. Minor differences on fermentation profile were observed between AMY and ISO for WPCS and earlage. An interaction between hybrid and storage length was observed for DM losses in WPCS, where losses were similar at 30, 60 and 90 d, but lower for AMY compared with ISO at 120 d. No effect of hybrid was observed on yeast and mold counts for WPCS or earlage. The aerobic stability of WPCS was greater for AMY than ISO. For earlage, AMY had greater DM losses and aerobic stability than ISO. An interaction between hybrid and storage length was observed for ammonia-N in both WPCS and earlage, where ammonia-N was similar at 0 d but greater for AMY than ISO throughout later storage lengths. A similar interaction was observed for water-soluble carbohydrates (WSC) concentrations in WPCS, where ISO had greater WSC than AMY at 0 d but was similar throughout later storage lengths. However, AMY earlage had a greater WSC concentration throughout storage length, but a lesser magnitude after ensiling. Starch concentration was greater for AMY than ISO in WPCS and earlage. Greater starch disappearances at 0 h and 6 h were observed for ISO in WPCS and earlage. Minor effects on fermentation profile, microbial counts, aerobic stability and nutrient composition suggests that AMY can be ensiled for prolonged periods with no concerns for undesirable fermentation or nutrient losses. However, in situ starch disappearance was lower for AMY compared with ISO. (The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).) |
| فهرسة مساهمة: | Keywords: aerobic stability; amylase; corn hybrid; starch disappearance |
| المشرفين على المادة: | EC 3.2.1.1 (alpha-Amylases) 9005-25-8 (Starch) |
| تواريخ الأحداث: | Date Created: 20240126 Date Completed: 20240522 Latest Revision: 20240530 |
| رمز التحديث: | 20240530 |
| DOI: | 10.3168/jds.2023-24228 |
| PMID: | 38278297 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3198 |
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| DOI: | 10.3168/jds.2023-24228 |