دورية أكاديمية
Theileria annulata subtelomere-encoded variable secreted protein-TA05560 interacts with bovine RNA binding motif protein 39 (RBM39).
| العنوان: | Theileria annulata subtelomere-encoded variable secreted protein-TA05560 interacts with bovine RNA binding motif protein 39 (RBM39). |
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| المؤلفون: | Ma Q; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China. Electronic address: maquanying2004236@126.com., Han Y; State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Animal Disease Pathogen Diagnosis and Green Prevention and Control Technology of Qinghai Province, Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China., Fu Y; State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Animal Disease Pathogen Diagnosis and Green Prevention and Control Technology of Qinghai Province, Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China., Teng G; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China., Wang X; School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, China., Liu J; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, China., Li Z; State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Animal Disease Pathogen Diagnosis and Green Prevention and Control Technology of Qinghai Province, Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, China. Electronic address: lizhi19880717@163.com. |
| المصدر: | Acta tropica [Acta Trop] 2024 Apr; Vol. 252, pp. 107133. Date of Electronic Publication: 2024 Jan 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Theileria annulata*/genetics , Theileria* , Theileriasis*/parasitology, Cattle ; Animals ; Humans ; HEK293 Cells ; Proteins/metabolism ; B-Lymphocytes ; RNA-Binding Motifs |
| مستخلص: | Theileria annulata is the only eukaryotic pathogen able to transform bovine leukocytes, including B cells, macrophages and dendritic cells. T. annulata-transformed cells exhibit several cancer-like phenotypes, such as hyperproliferation, immortalization and dissemination. Although several parasite factors involved in bovine cell transformation have been explored, the roles of subtelomere-encoded variable secreted proteins (SVSPs) of the parasite in host-cell interactions are largely unknown. In the present study, the target molecule TA05560, a member of the SVSP multigene family of T. annulata, was identified at the mRNA level during different life cycles through a quantitative real-time PCR assay, and the subcellular distribution of TA05560 was examined via confocal microscopy. The results showed that the parasite molecule TA05560 was transcribed mainly in the schizont stage of T. annulata infection, and the protein was distributed in the nucleus and cytoplasm of the parasitized cells. The potential host cell proteins that interact with TA05560 were screened using the yeast two-hybrid system, and the direct interaction between TA05560 and its prey protein, Bos taurus RNA binding motif protein 39 (RBM39) was further identified in HEK293T cells by using confocal microscopy, coimmunoprecipitation and bimolecular fluorescence complementation assays. Moreover, the interaction between TA05560 and its host protein was observed in T. annulata-infected cells via confocal microscopy. Therefore, our study is the first to show that the T. annulata-secreted TA05560 protein directly binds to both the exogenous and endogenous host cell molecule RBM39, laying the foundation for exploring host-parasite interactions and understanding the transformation mechanisms induced by T. annulata and other transforming parasites. Competing Interests: Declaration of competing interest The authors declare that they have no competing conflict of interests. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Schizont stage; Subtelomere-encoded variable secreted protein; TaNM1 cells; Theileria annulata |
| المشرفين على المادة: | 0 (Proteins) |
| تواريخ الأحداث: | Date Created: 20240127 Date Completed: 20240306 Latest Revision: 20240306 |
| رمز التحديث: | 20240306 |
| DOI: | 10.1016/j.actatropica.2024.107133 |
| PMID: | 38280638 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2024.107133 |